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Fibroblast growth factor receptor 3 (FGFR3), also known as CD333, is a tyrosine kinase receptor located on the cell membrane and belongs to the FGFR family. The FGFR3 gene is located on human chromosome 4 at p16.3 and contains 19 exons, expressing several slightly different isoforms. FGFR3 protein structure comprises an extracellular structure with 2 or 3 immunoglobulin-like domains, a transmembrane domain and a cytoplasmic tyrosine kinase domain. When the extracellular portion binds with FGF, FGFR3 dimerizes and autophosphorylates and then triggers a downstream signaling cascade that ultimately affects cell growth, migration, angiogenesis, and differentiation.
FGFR3 mutations are often closely related to diseases, including human genetic diseases (Table 1) and cancer. Studies have shown that FGFR3 can regulate ossification and thus affect bone growth, and the over-activation of FGFR3 can lead to achondroplasia, a dominant hereditary disease. In addition, overexpression of FGFR3 provides an oncogenic signal. In bladder cancer, FGFR3 appears post-translational modifications that do not occur in normal cells, suggesting that FGFR3 may be a potential target for the treatment of bladder cancer. FGFR3 inhibitors have been used in cancer treatment in many early clinical trials, such as BGJ398 for urothelial cancer. Studies on the tyrosine kinase signaling pathways mediated by FGFR3 in cell proliferation, drug resistance against anticancer drugs, and other cellular activities have played an important role in promoting bioscience research and new drugs development.
Table 1: Mutations in FGFR3 associated with disease in Humans (and Mice) (Ornitz DM. et al., 2015)
|Gene Name||Mutation||Associated Disease|
|FGFR3||Missense Mutation||Hypochondroplasia, Achondroplasia, Thanatophoric dysplasia, Coronal craniosynostosis, Crouzon syndrome with acanthosis nigricans, Platyspondylic lethal skeletal dysplasia, Achondroplasia with developmental delay, (SADDAN), Muenke syndrome, Saethre-Chotzen-syndrome, CATSHL syndrome, Achondroplasia.|
FGFR3 Gene Editing Service
CRISPR/Cas9 PlatformCB, one of the leading genomic editing companies, is specialized in providing a set of services for CRISPR-Cas9 genome editing. We have national labs and a team of professional scientists from world-class universities. Based on our platform, we can provide you the most professional and comprehensive CRISPR/Cas9 gene-editing cell lines or animal models to meet your specific project needs and provide experienced scientific support at every step.
CRISPR/Cas9 PlatformCB has successfully implemented gene editing of FGFR3 in a variety of cell lines, including easy-to-transfect cell lines and hard-to-transfect cells. Our full cell line generation services cover from sgRNA design/construction to final cell line generation/verification. We also provide you with one-stop-shop custom FGFR3 gene-editing services in the cell line you desire, including point mutation and gene insertion, conditional knockout/knockin. Our FGFR3 gene editing cell line generation services include:
✧ SgRNA design and synthesis
✧ Transfect the cell line you interest
✧ Select the high expression cell and sort monoclonal cell
✧ Validate the knockout/knockin/point mutation of FGFR3 by PCR and sequencing
✧ Produce cryogenic preserved vials of stable cells and a final report
Typically, we develop CRISPR-mediated gene-editing cell lines, including HEK239T, Hela, HepG2, U87, but we can use other cell lines according to your requirements.
Host cell line: Ba/F3, CHO, MDA-MB-453, MDA-MB-231NIH3T3, T47D, Neuro2a, MCF7, RKO, K562, RAW264.7, etc.
CRISPR/Cas9 PlatformCB also has extensive experience in manipulating animal embryos and been very successful in incorporating CRISPR-Cas9 technology into our program, which have been well recognized by our customers. We have successfully obtained >300 mouse models with very high efficiency and success rate by using CRISPR/Cas9-mediated genome editing technology. And we can provide you with CRISPR/Cas FGFR3 gene-editing animal models at a reasonable cost and in a shorter time. Our FGFR3 gene editing animal model generation services include:
➢ FGFR3 gene conventional knockout animals
➢ FGFR3 gene conditional knockout animals
➢ FGFR3 point mutation animals
➢ FGFR3 knockin animals
Alternative species: mouse, rat, rabbit, zebrafish, C. elegans, etc.
CRISPR/Cas9 PlatformCB is professional in providing custom genome engineering projects. Tell us your needs, we will provide you with a practical CRISPR/Cas gene-editing strategy and design. And we guarantee our clients the most reliable and efficient research services to best match your research goals. If you don't see the gene editing service related to FGFR3 you need above, don't hesitate to contact us.
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