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FGFR2, also named CD332, is a transmembrane protein and belongs to the fibroblast growth factor receptors family (FGFR family), which are mitogenic signaling molecules that have roles in important processes such as cell division, regulation of cell growth and mutation, the formation of blood vessels, wound healing, and embryonic development. FGFR2 is a transmembrane catalytic receptor with intracellular tyrosine kinase activity, similar to FGFRs. The FGFR2 gene is located on human chromosome 10 and expresses two slightly different subtypes: FGFR2IIIb, which is mainly expressed in epithelial cells, and FGFR2IIIc, which is mainly expressed in mesenchymal cells.
When bound to a ligand, FGFR2 activates a series of intracellular signaling pathways, including the RAS-MAPK pathway, the PI3K/AKT pathway. FGFR2 signal is down-regulated by ubiquitination, internalization, and degradation. Studies have shown that mutations in FGFR2 are associated with many diseases such as Apert syndrome, Crouzon syndrome, Jackson-Weiss syndrome, Pfeifer syndrome, and so on (table 1). In addition, changes in FGFR2 gene activity may promote tumorigenesis and metastasis by promoting cell proliferation, cell movement, and neovascular development that nourishes growth tumors. For example, FGFR2 gene expression is elevated in certain types of gastric cancer, which is associated with poor disease prognosis. Changes in FGFR2 gene expression are also associated with ovarian, breast, cervical, pancreatic, and head and neck cancers. Researches on inhibitors of FGFR2 signaling are expected to provide new directions and ideas for new drug development, especially targeted drugs for cancer therapy.
Table 1: Acquired and Heritable Mutations in FGFR2 in human diseases (Ornitz DM. et al., 2015)
|Gene Name||Mutation||Associated Disease|
|FGFR2||Missense Mutation||Apert syndrome, Crouzon syndrome, Jackson-Weiss syndrome, Pfeifer syndrome, non-syndromic craniosynostosis, Bent bone dysplasia|
FGFR2 Gene Editing Service
CRISPR/Cas9 PlatformCB is one of the leading-edge experts among the frontier of CRISPR-Cas9 genome editing technology, and specializes in providing a set of custom services for CRISPR-Cas9 genome editing. We can provide you the most professional and comprehensive FGFR2 gene editing cell lines or animal models to meet your specific project needs and provide experienced scientific support at every step.
Based on our platform and our professional scientists, CRISPR/Cas9 PlatformCB has successfully implemented gene editing of FGFR2 in a variety of cell lines, such as HEK239T, Hela, HepG2, U87 and other cancer and stem cells. Our full cell line generation services cover from sgRNA design/construction to final cell line generation/verification. We also provide you with a one-stop service for custom FGFR2 gene-editing services in the cell line you desire, including point mutation and gene insertion, conditional knock-out/knock-in. Our FGFR2 gene editing cell line generation services include:
✧ SgRNA design and synthesis
✧ Transfect the cell line you interest
✧ Select the high expression cell and sort monoclonal cell
✧ Validate the knockout/knockin/point mutation of FGFR2 by PCR and sequencing
✧ Produce cryogenic preserved vials of stable cells and a final report
Host cell line: Ba/F3, CHO, HEK293, HEK239T, Hela, MDA-MB-453, MDA-MB-231NIH3T3, T47D, Neuro2a, HepG2, MCF7, RKO, K562, RAW264.7, U87 etc.
CRISPR/Cas9 PlatformCB also has extensive experience in manipulating animal embryos and been very successful in incorporating CRISPR-Cas9 technology into our program, which have been well recognized by our customers. Through our technology and platform, we can produce FGFR2 transgenic and gene-targeted animals at a reasonable cost in a shorter time. Our FGFR2 gene editing animal model generation services include:
➢ FGFR2 gene conventional knockout animals
➢ FGFR2 gene conditional knockout animals
➢ FGFR2 point mutation animals
➢ FGFR2 knockin animals
Alternative species: mouse, rat, rabbit, zebrafish, C. elegans, etc.
CRISPR/Cas9 PlatformCB is professional in providing custom genome engineering projects. Based on national advanced laboratory platforms, professional team and efficient operation, we guarantee our clients the most reliable and efficient research services to best match your research goals. If you don't see the gene editing service related to FGFR2 you need above, don't hesitate to contact us.
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