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FGFR1 (Fibroblast growth factor receptor 1), also known as fms-related tyrosine kinase-2/Pfeiffer syndrome, is a member of the fibroblast growth factor receptor (FGFR) family. FGFR family is cell surface membrane receptor that possesses tyrosine kinase activity. When combined with appropriate FGF, FGFR1 elicits cellular responses such as proliferation, migration, and differentiation by activating a series of intracellular signaling pathways including Ras subfamily/ERK, PKC, phospholipase C/PI3K/AKT, Ip3-induced raising of cytosolic Ca2+, Ca2+/calmodulin-activated elements and pathways.
Studies have shown that mutations in FGFR1 are associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, Osteoglobulin dysplasia, and autosomal dominant Kallmann syndrome2. And chromosomal aberrations involving FGFR1 are associated with stem cell myeloproliferative disorders and stem cell leukemia lymphoma syndrome. At the same time, FGFR1 also has a certain correlation with tumors (Table 1). FGFR1 promotes inflammation via activating NF-κB signaling in the tumor microenvironment. Due to the importance of FGFR1 in cell life activities, studies on the molecular mechanism of FGFR1 in the pathogenesis of cancer and the studies of FGFR1 as a therapeutic target FGFR1 are still hot research topics in life sciences.
Table 1: Acquired and Heritable Mutations in FGFR1 in Malignancy (Ornitz DM. et al., 2015)
|Gene Name||Mutation||Associated Disease|
|FGFR1||Amplification||Small cell lung cancer, Squamous cell lung cancer, Breast cancer, Ovarian cancer, Pancreatic ductal adenocarcinoma, Tongue squamous cell carcinoma|
|Missense mutation||Melanoma, Pilocytic astrocytoma|
|Translocation||Leukemia, Lymphoma, Alveolar rhabdomyosarcoma, Glioblastoma, Myeloproliferative syndrome (fusion with CUX1, FGFROP2, FIM, RANBP2/NUP358, SQSTM1, TRP, ZNF198)|
FGFR1 Gene Editing Service
CRISPR/Cas9 PlatformCB, one of the leading-edge experts among the frontier of CRISPR-Cas9 genome editing technology, specializes in providing a set of custom services for CRISPR-Cas9 genome editing. To explore the mechanisms through which FGFR1 elicits cellular biology progress especially related to cancers, we can provide you the most professional and comprehensive FGFR1 gene editing cell lines or animal models to meet your specific project needs and provide experienced scientific support at every step.
Based on our platform, CRISPR/Cas9 PlatformCB has successfully implemented gene editing of FGFR1 in a variety of cell lines, including easy-to-transfect cell lines and hard-to-transfect cells. Our full cell line generation service cover from sgRNA design/construction to final cell line generation/verification. We also provide you with one-stop services for custom FGFR1 gene editing cell line you desire generation service, including point mutation and gene insertion, conditional knock-out/knock-in. Our FGFR1 gene editing cell line generation services include:
✧ SgRNA design and synthesis
✧ Transfect the cell line you interest
✧ Select the high expression cell and sort monoclonal cell
✧ Validate the knockout/knockin/point mutation of FGFR1 by genomic PCR
✧ Produce cryogenic preserved vials of stable cells and a final report
Host cell line: Ba/F3, CHO, HEK293, HEK239T, Hela, MDA-MB-453, MDA-MB-231NIH3T3, T47D, Neuro2a, HepG2, MCF7, RKO, K562, RAW264.7, etc.
As CRISPR enabled targeted genome editing in a simple, efficient, and economical manner, the process of creating transgenic animals became simpler. CRISPR/Cas9 PlatformCB has extensive experience in manipulating animal embryos and been very successful in incorporating CRISPR-Cas9 technology into our program, which have been well recognized by our customers. Based on our research platform and team of scientists, we can produce FGFR1 transgenic and gene-targeted animals at a reasonable cost in a shorter time. Our FGFR1 gene editing animal model generation services include:
➢ FGFR1 gene conventional knockout animals
➢ FGFR1 gene conditional knockout animals
➢ FGFR1 point mutation animals
➢ FGFR1 knockin animals
Alternative species: mouse, rat, rabbit, zebrafish, C. elegans, etc.
As one of the leading-edge experts among the frontier of CRISPR-Cas9 genome editing technology, CRISPR/Cas9 PlatformCB is professional in providing custom genome engineering projects. Based on national advanced laboratory platforms, professional team and efficient operation, we guarantee our clients the most reliable and efficient research services to best match your research goals. If you don't see the gene editing service related to FGFR1 you need above, don't hesitate to contact us.
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