EphA2 Gene Editing

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EphA2 Gene Editing    

Ephrin type-A receptor 2 (EphA2) is a 130 kDa glycoprotein receptor and belongs to the largest EPH family receptor tyrosine kinases. The EphA2 protein structure has an intracellular kinase domain, and an extracellular domain with an Ephrin binding domain and two fibronectin type III repeats. Binding to Eph triggers a series of intracellular signals including RAS/MAPK, FAK/SRC, ABL, RHO/RAC/CDC42, and PI3K/AKT/mTOR, and regulates migration, proliferation, integrin-mediated adhesion and differentiation of cells (Figure 1).

EphA2 signaling is involved in the regulation of many development-related events, especially in neurodevelopment. EphA2 is also involved in the regulation of lens formation, and mutations in the EphA2 gene are responsible for hereditary cataracts. In addition, EphA2 plays an important regulatory role in malignant transformation, angiogenesis, and metastasis of normal cells, and EphA2 is overexpressed in a variety of cancer cells, including glioblastoma, colorectal cancer, gastric cancer, breast cancer, thyroid cancer, ovarian cancer, endometrial cancer, cervical cancer, pancreatic cancer, prostate cancer, melanoma, bladder cancer, kidney cancer, lung cancer, and liver cancer. In cancer therapy, EphA2 may affect prognosis due to its ability to promote cell migration and invasion. EphA2 may provide new clues to better molecular understanding of neurodegenerative diseases and cancer development, and further research on EphA2 as a new prognostic biomarker and the therapeutic target will provide new support for cancer treatment.

 Structure and Function of the EphA2 Canonical PathwayFigure 1: Structure and Function of the EphA2 Canonical Pathway (Yue Zhou, 2017)

EphA2 Gene Editing Service

As a leading genomic editing company, CRISPR/Cas9 PlatformCB is specialized in providing a set of services for CRISPR-Cas9 genome editing. We can provide you custom CRISPR/Cas9 gene-editing services from strategy design to genetic edited cell lines or animal models to meet your specific project needs and provide experienced scientific support at every step.

  • EphA2 Gene Editing Cell Line Generation

CRISPR/Cas9 PlatformCB has successfully implemented gene editing of EphA2 in a variety of cell lines, including easy-to-transfect cell lines and hard-to-transfect cells. Our full cell line generation services cover from sgRNA design/construction to final cell line generation/verification. Our EphA2 gene editing cell line generation services include:

✧ SgRNA design and synthesis
✧ Transfect the cell line you interest
✧ Select the high expression cell and sort monoclonal cell
✧ Validate the knockout/knockin/point mutation of EphA2 by PCR and sequencing
✧ Produce cryogenic preserved vials of stable cells and a final report

Typically, we develop CRISPR-mediated gene-editing cell lines, including HEK239T, Hela, HepG2, U87, but we can use other cell lines according to your requirements.

Host cell line: Ba/F3, CHO, MDA-MB-453, MDA-MB-231NIH3T3, T47D, Neuro2a, MCF7, RKO, K562, RAW264.7 etc.

  • EphA2 Gene Editing Animal Model Generation

CRISPR/Cas9 PlatformCB also has extensive experience in incorporating CRISPR-Cas9 technology into animal models, which have been well successful and recognized by our customers. We have obtained >300 mouse models with very high efficiency and success rate by using CRISPR/Cas9-mediated genome editing technology. Based on our platform, we can provide you with CRISPR/Cas EphA2 gene-editing animal models at a reasonable cost and in a shorter time. Our EphA2 gene editing animal model generation services include:

➢ EphA2 gene conventional knockout animals
➢ EphA2 gene conditional knockout animals
➢ EphA2 point mutation animals
➢ EphA2 knockin animals

Alternative species: mouse, rat, rabbit, zebrafish, C. elegans, etc.

If you don't see the gene editing service related to EphA2 you need above, don't hesitate to contact us. CRISPR/Cas9 PlatformCB is professional in providing custom CRISPR/Cas genome engineering projects. Tell us your needs, we will provide you with a professional genetic edit strategy and design. We guarantee our clients the most reliable and efficient research services to best match your research goals.

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References:

  1. Thomas Neill. el at. EphA2 is a functional receptor for the growth factor progranulin. JCB. 2016. 215 (5): 687.
  2. Philip D. Dunne. el at. EphA2 Expression Is a Key Driver of Migration and Invasion and a Poor Prognostic Marker in Colorectal Cancer. Biology of Human Tumors. 2016. 22 (1).
  3. Dana M. Brantley-Sieders. el at. EphA2 receptor tyrosine kinase regulates endothelial cell migration and vascular assembly through phosphoinositide 3-kinase-mediated Rac1 GTPase activation. Journal of Cell Science. 2004 117: 2037-2049.
  4. Yue Zhou and Hiroaki Sakurai. Emerging and Diverse Functions of the EphA2 Noncanonical Pathway. Biol. Pharm. Bull. 2017. 40, 1616–1624.
For research use only. Not intended for any clinical use.

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