DLL1 Gene Editing

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DLL1 Gene Editing    

DLL1 (similar to typical Notch ligand 1) is an active Notch ligand that belongs to the Delta family. Notch signaling is an evolutionarily conserved signaling pathway that plays a central role in determining cell fate and maintaining tissue homeostasis in various organs during embryonic development and postnatal life.

During mouse fetal development, DLL1 activates the Notch1 pathway to maintain arterial identity in the aorta. The DLL1-Notch signal induces the proliferation of osteoblast progenitor cells, thereby expanding the osteoblast bank. In addition, DLL1-Notch signaling regulates the coupling of osteoblasts and osteoclasts by controlling the differentiation of osteoblast progenitor cells into mature osteoblast/osteocytes, thereby maintaining bone integrity.

Maintenance of arterial identity mediated by Dll1-Notch signaling.Figure 1: Maintenance of arterial identity mediated by Dll1-Notch signaling. (Tsutomu Kume. 2009)

Genetic studies have shown that DLL1-Notch signaling is important for tumor cell proliferation, angiogenesis, and cancer stem cell function in breast cancer. Loss of DLL1 inhibits tumor growth and lung metastasis of intraluminal breast cancer. However, estrogen signaling can stabilize DLL1 protein by preventing its proteasome and lysosome degradation, while inhibiting the ubiquitination of DLL1.

In addition, Dll1 is also involved in promoting tumor-initiating cells/cancer stem cells in a variety of cancers such as glioblastoma, renal cell carcinoma, and rhabdomyosarcoma, and enhancing T cell-mediated anti-tumor immunity.

DLL1 Gene Editing Service

CRISPR/Cas9 PlatformCB provides you with comprehensive CRISPR/Cas9 gene editing services and products to a wide range of genomics researchers. As a leading biotechnology company specializing in gene editing, we can help you effectively regulate your target genes editing using the CRISPR/Cas9 system.

  • Our DLL1 gene editing services include

Mutation via CRISPR Gene Editing

We are able to perform specific DNA deletions, mutations or substitutions to study gene function with CRISPR/Cas9 gene editing technology with high precision. Available services:
➢ Knockout
➢ Point Mutation
➢ Conditional knockout/knock-in
➢ Floxed allele insertion

Mark by CRISPR gene editing

Using the CRISPR/Cas9 gene editing technology, CRISPR/Cas9 PlatformCB is able to tag your genes at the endogenous locus and visualize them with fluorescent proteins or immune-tags for biochemical studies. Available services:
➢ Fluorescent tag
➢ Immuno-tags
➢ Custom tags and combinations are also available

Project WorkflowModel Types
Strategy designBlood Lineage Cells
gRNA design and synthesis, donor DNA design and synthesis (if needed)Cancer Cell Lines
CRISPR/Cas9 vector constructionStem Cells
Transfection into the cells or microinjection into embryo single cellOther Cell Lines
Validate by PCR and sequencingAnimal models
Delivery models and the final report 

CRISPR/Cas9 PlatformCB is able to adapt the solution to your detailed requirements from the early strategic design to the final model. We guarantee to provide our customers with excellent service. If you have any questions, please feel free to contact us.

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References

  1. Huang Y. et al. Resuscitating cancer immunosurveillance: selective stimulation of DLL1-Notch signaling in T cells rescues T-cell function and inhibits tumor growth. Cancer Res. 2011; 71:6122-31.
  2. Inga Sörensen. et al. DLL1-mediated Notch activation regulates endothelial identity in mouse fetal arteries. Blood. 2009; 113(22):5680-5688.
  3. Rumela Chakrabarti. et al. Notch ligand Dll1 mediates cross-talk between mammary stem cells and the macrophageal niche. Science. 2018; 360(6396):4153.
  4. Sushil Kumar. et al. Estrogen-dependent DLL1-mediated Notch signaling promotes luminal breast cancer. Oncogene. 2019; 38:2092-2107.
  5. Tsutomu Kume. Novel insights into the differential functions of Notch ligands in vascular formation. J Angiogenes Res. 2009; 1: 8.
  6. Xiao W. et al. Notch signaling plays a crucial role in cancer stem-like cells maintaining stemness and mediating chemotaxis in renal cell carcinoma. J Exp Clin Cancer Res. 2017; 36:41.
  7. Yukari Muguruma. et al. Maintenance of Bone Homeostasis by DLL1- Mediated Notch Signaling. J Cell Physiol. 2017; 232(9):2569-580.
For research use only. Not intended for any clinical use.

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