DDR2 Gene Editing

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DDR2 Gene Editing    

DDR2 (Discoidin domain-containing receptor 2), also named CD167b, is a transmembrane receptor tyrosine kinase (RTK) and belongs to the DDR family. The DDR2 gene is located at human chromosome 1 at position 23.3 and expressed in a variety of cell types. DDR2 is a collagen-induced receptor that binds to the DDR2 extracellular discoidin domain, which leads to DDR2 phosphorylation. Activation of DDR2 activates a variety of intracellular signal transduction pathways involved in cell adhesion, proliferation, and extracellular matrix remodeling. DDR2 is able to interact with SHC1 and phosphorylate Shp2. DDR2 interacts with collagen by a variety of intracellular signal transduction pathways. In the absence of collagen-binding and SRC phosphorylation, DDR2 is in an inactive state. Tyrosine phosphorylation enhances ATP affinity and catalytic activity.

Studies have shown that DDR2 may be involved in wound repair and regulation of tumor growth and invasiveness. At the same time, DDR2 abnormalities are associated with many diseases, including fibrosis and cancer, and DDR2 mutations are the cause of short-limb hand-type vertebral dysplasia.

DDR2 Gene Editing Service

CRISPR/Cas9 PlatformCB is one of the leading genomic editing companies with national-class labs, a team of professional scientist from world-class universities. We are specialized in providing a set of CRISPR/Cas9 gene editing services from strategy design to final cell lines or animal models to meet your specific project needs and provide experienced scientific support at every step.

  • DDR2 Gene Editing Cell Line Generation

Our scientists are very familiar with the culture methods and genetic background of various cell lines, and have successfully implemented gene editing of DDR2 in both easy-to-transfect cell lines and hard-to-transfect cells. Tell us the cell lines your project needs, we will offer you a one-stop-shop DDR2 gene editing services including knockout, knockin, point mutation. And our DDR2 gene editing cell line generation services include:

✧ SgRNA design and synthesis
✧ Transfect the cell line you interest
✧ Select the high expression cell and sort monoclonal cell
✧ Validate the knockout/knockin/point mutation of DDR2 by PCR and sequencing
✧ Produce cryogenic preserved vials of stable cells and a final report

Typically, we develop CRISPR-mediated gene-editing cell lines, including HEK239T, Hela, HepG2, U87, but we can use other cell lines according to your requirements.

Host cell line: Ba/F3, CHO, MDA-MB-453, MDA-MB-231NIH3T3, T47D, Neuro2a, MCF7, RKO, K562, RAW264.7, etc.

  • DDR2 Gene Editing Animal Model Generation

CRISPR/Cas9 PlatformCB also has extensive experience in incorporating CRISPR-Cas9 technology into animal models. We have successfully obtained >300 mouse models with very high efficiency rate via CRISPR/Cas9-mediated genome editing technology. We can provide you with CRISPR/Cas DDR2 gene-editing animal models at a reasonable cost and in a shorter time. Our DDR2 gene editing animal model generation services include:

➢ DDR2 gene conventional knockout animals
➢ DDR2 gene conditional knockout animals
➢ DDR2 point mutation animals
➢ DDR2 knockin animals

Alternative species: mouse, rat, rabbit, zebrafish, C. elegans, etc.

CRISPR/Cas9 PlatformCB is professional in providing custom CRISPR/Cas genome engineering projects. If you don't see the gene editing service related to DDR2 you need above, don't hesitate to contact us. We guarantee our clients the most reliable and efficient research services to best match your research goals, and faster turnaround times, lower prices.

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References:

  1. Leitinger B. "Transmembrane collagen receptors". Annu. Rev. Cell Dev. Biol. 2011. 27: 265–90.
  2. Fu HL. et al. Discoidin domain receptors: unique receptor tyrosine kinases in collagen-mediated signaling. J. Biol. Chem. 2013. 288 (11): 7430–7.
  3. Ikeda K. et al. Discoidin domain receptor 2 interacts with Src and Shc following its activation by type I collagen". J. Biol. Chem. 2002. 277 (21).
  4. Iwai LK. et al. Phosphoproteomics of collagen receptor networks reveals SHP-2 phosphorylation downstream of wild-type DDR2 and its lung cancer mutants. Biochem. J. 2013. 454 (3): 501–13.
  5. Xu H. et al. (Dec 2012). "Discoidin domain receptors promote α1β1- and α2β1-integrin mediated cell adhesion to collagen by enhancing integrin activation". PLoS ONE. 207 (12).
For research use only. Not intended for any clinical use.

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