CXCR4 Gene Editing

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CXCR4 Gene Editing    

CXCR4 (CXC Chemokine Receptor Type 4), also named as CD184, is a member of the evolutionarily highly conserved GPCR family and belongs to the seven-TM GPCR. CXCR4 is one of the most studied chemokine receptors because it was first discovered as a co-receptor for HIV entry. CXCR4 is the major receptor type of CXCL12. CXCL12/CXCR4 chemokine signaling plays a key role in regulating various neuronal developmental processes and regulating synaptic plasticity. The binding of CXCL12 to CXCR4 initiates various downstream signaling pathways leading to excessive responses, such as intracellular calcium increase, gene transcription, chemotaxis, cell survival, and proliferation. Recent studies have shown that extracellular ubiquitin can also act as an immune-modulator through CXCR4-mediated signaling.

CXCR4 is overexpressed in more than 23 different types of human cancers and has been identified as a poor prognostic biomarker which helps tumor growth, angiogenesis, metastasis, and therapeutic resistance, including kidney cancer, lung cancer, brain cancer, prostate cancer, breast cancer, pancreatic cancer, ovarian cancer, and melanoma. The different roles of CXCR4 in different types of cancer, as well as HIV infection and other pathological conditions, have made CXCR4 an important target for therapeutic intervention.

Figure 1: Schematic diagram of CXCR4/CXCL12 signaling pathway (Samit Chatterjee. 2014)

CXCR4 Gene Editing Service

CRISPR/Cas9 PlatformCB, one of the global leading biotechnological companies specializing in gene editing, is dedicated to offering comprehensive CRISPR/Cas9 gene editing services and products to a wide range of genomics researchers. Based on our platform, we can help you effectively control target genes deleted, inserted or point mutated in cells or animals via CRISPR/Cas9 technology.

  • CXCR4 Gene Editing Cell Line Generation

We have successfully implemented CXCR4 CRISPR/Cas9 gene edited in both easy-to-transfect cell lines and hard-to-transfect cells. With deep gene editing knowledge and extensive experience in experimental operation and data processing, we will offer you full-length custom CXCR4 gene editing service from strategy design to final stable cells. Our CXCR4 gene editing cell line generation services include:

➢ gRNA design and synthesis
➢ Transfect the cell lines you're interested
➢ Select the high expression cells and sort monoclonal cell
➢ Validate the knockout/knockin/point mutation of CXCR4 by PCR and sequencing
➢ Produce cryogenic preserved vials of stable cells and a final report

Typically, we develop CRISPR-mediated gene editing cell lines including HEK239T, Hela, HepG2, U87, but we can use other cell lines according to your requirements.

Other host cell lines available: Ba/F3, CHO, MDA-MB-453, MDA-MB-231NIH3T3, T47D, Neuro2a, MCF7, RKO, K562, RAW264.7, etc.

  • CXCR4 Gene Editing Animal Model Generation

CRISPR/Cas9 PlatformCB also has extensive experience in incorporating CRISPR/Cas9 technology into animal models, which have been fully recognized by our clients. Tell us your projects’ needs, we provide a one-stop-shop CXCR4 CRISPR/Cas9 gene editing animal service and guarantee at least 2 founders or 3 F1 animals with shorter turnaround time and lower price. Our CXCR4 gene editing animal model generation services include:

➢ CXCR4 gene conventional knockout animals
➢ CXCR4 gene conditional knockout animals
➢ CXCR4 point mutation animals
➢ CXCR4 knockin animals

Alternative species: mouse, rat, rabbit, zebrafish, C. elegans, etc.

With many years of experience on gene editing, CRISPR/Cas9 PlatformCB provides the best gene editing services and products for academic research, biotech research and pharmaceutical drug discovery with excellent quality management and quality assurance capacity. To accelerate the achievement of your research goals, our gene editing expert team provides you with custom CRISPR/Cas9 services for any specific gene to help you solve problems encountered during your research. If you have any question, please feel free to contact us.

Related Products at CRISPR/Cas9 PlatformCB

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References

  1. Tommaso Pozzobona. et al. CXCR4 signaling in health and disease. Immunology. 2016; 177:6-15.
  2. Samit Chatterjee. et al. The Intricate Role of CXCR4 in Cancer. Adv Cancer Res. 2014; 124:31-82.
  3. Peter J. Richardson. et al. CXCR4 and Glioblastoma. Anti-Cancer Agents in Medicinal Chemistry. 2016; 16(1):59-74.
  4. Hongyan Li and Rong Wang. A focus on CXCR4 in Alzheimer's disease. Brain Circ. 2017 Oct-Dec; 3(4): 199-203.
  5. Jan A. Burger & Thomas J. Kipps. CXCR4: a key receptor in the crosstalk between tumor cells and their microenvironment. Blood. 2006; 107:1761-1767.
For research use only. Not intended for any clinical use.

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