CRISPR Rabbits for Human Papillomavirus Research    

Approximately 5% of human cancers can be attributed to human papillomavirus (HPV) infection. Despite the availability of prophylactic HPV vaccines, HPV-related diseases and cancers remain a significant public health and economic burden worldwide. Since its use as a preclinical model of HPV infection, the Cottontail rabbit papillomavirus (CRPV) rabbit model has been widely used to study virus-host interactions and has played a key role in the successful development of three prophylactic virus-like drugs for particle vaccines. These preclinical models have proven valuable for understanding the pathogenesis of high-risk papillomavirus infection and cancer development. In addition, rabbit models have been widely used to test antiviral and antitumor therapies. These rabbit models will continue to add to our understanding of viral pathogenesis, immunology, and cancer development mechanisms.

Fig. 1 Discovery of Sylvalagus floridanus papillomavirus 1 (SfPV1) and the development of the SfPV1/domestic rabbit model. (Cladel NM, et al., 2019)

Solution

CRISPR/Cas9 Platform^CB uses the CRISPR/Cas9 system to perform knockouts in rabbits, targeting knockout genes including, but not limited to, Foxn1, Il2rg, and Rag2. Rag2 is involved in the V(D)J recombination process of B and T cells and is essential for the production of mature B and T lymphocytes. Therefore, Rag2-deficient patients also frequently suffer from SCID. these immunodeficient rabbit lines will help contribute to the development of therapeutic strategies in the CRPV rabbit model.

What Can We Help You in Human Papillomavirus Research?

• Development of preventive and therapeutic vaccines: development of preventive and therapeutic vaccines targeting early-stage and late-stage genes.
• Studying virus-host interactions: investigating the pathogenesis of SfPV1 and the host immune response to SfPV1 infection.
• Analyzing gene function: dissecting the function of different genes and their role in the pathogenesis of papillomavirus-associated skin infections.
• Testing antiviral compounds and developing new therapies for HPV-associated diseases and cancers.

Advantages of Our Technology

• Compared with traditional gene targeting methods, it greatly shortens the development cycle.
• Break the restriction on rabbit genetic strains to enable gene editing on the basis of different genetic backgrounds or on the basis of existing genetically modified rabbit models.
• Reduce the customization cost of knockout and knock-in rabbit models.

Related Services

• Custom Rabbit Model

Related Products

• CRISPR/Cas9 Kits
• Cas9 Protein
• Cas9 mRNA
• Cas9 Related Plasmids
• Pre-made Cas9 Virus Particles

CRISPR/Cas9 Platform^CB collaborates with leading researchers to develop CRISPR technology for creating custom rabbit models. All you need to do is provide the model requirements and our technical team will complete the experimental protocol design, experimental protocol execution, construction of gene editing disease models, and delivery according to your needs. With our highly efficient rabbit knockout platform covering many target genes, and with our advanced technical capabilities, we can provide the right rabbit model to meet your drug discovery needs and advance disease research. If you are interested in our services, please feel free to contact us.