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CDK8 is a nuclear serine-threonine kinase that belongs to the cyclin-dependent kinase family and is involved in transcriptional regulation from yeast to mammals. CDK8 is a major SER2 kinase, and several CDK8 phosphorylation targets currently validated include RNA polymerase II (RNAPII) C-terminal domain (CTD), histone H3, subunits of general transcription factors (base transferases) and certain transactivators, However, how these phosphorylation events contribute to the overall biological activity of CDK8 remains unclear.
CDK8 as a transcription regulator
Figure 1: CDK8 function and its relationship with physiological (purple) and pathological (red) conditions (outer circle). (Ingeborg Menzl. 2019)
CDK8 and cancers
Since most of the CDK8-dependent pathways described are altered in cancer. CDK8 has been described as an oncogene for colorectal cancer (CRC) and several other cancer types, including melanoma and breast cancer. However, the exact mechanism by which CDK8 activity promotes cancer development remains to be elucidated. CDK8 has been shown to be a positive regulator of the transcriptional program that drives cancer cell proliferation and survival, including Wnt/β-catenin, ERK/MAP kinase signaling, and HIF1A. Therefore, by simultaneously reducing the activity of multiple carcinogenic pathways, the pharmacological inhibitory effect of CDK8 may have therapeutic value.
Targeting the therapeutic benefits of CDK8 in cancer
Figure 2: Potential therapeutic benefits of targeting CDK8 in cancer. (Ingeborg Menzl. 2019)
The dynamic association of CDK8 or CDK19 modules with mediators and the highly flexible composition of mediators may enhance or limit the potential of CDK8 as a drug target.
CDK8 Gene Editing Service
CRISPR/Cas9 PlatformCB has excellent quality management and quality assurance capabilities, providing the best gene-editing services and products for academic research, biotechnology research, and drug discovery. From early strategic design to the final model, we can adapt the solution to your detailed requirements. We guarantee to provide our customers with excellent service.
Our customized CDK8 gene editing services include
|gRNA design and synthesis, donor DNA design and synthesis (if needed)||CDK8 Gene Editing Cell Line Generation Service|
|CRISPR/Cas9 vector construction|
|Transfection into the cell lines you're interested|
|Select the high expression cells and sort monoclonal cell|
|Validate the right edited monoclonal cells by PCR and sequencing|
|Delivery the stable cells and a final report|
|gRNA design and synthesis, donor DNA design and synthesis (if needed)||CDK8 Gene Editing Animal Model Generation Service|
|CRISPR/Cas9 vector construction|
|Breed the founder mice, and validation|
|Delivery the F0 or F1 mice|
What kind of models we deal with?
|Blood Lineage Cells||RAW264.7, HMC1.2, K562, U937, etc.|
|Cancer Cell Lines||HEK293, HEK293T, Hela, MCF7, Neuro2a, HepG2, U87, etc.|
|Other Cell Lines||NIH3T3, MCF10, HEME, SW10, etc.|
|Animal models||Mouse, rat, rabbit, zebrafish, C. elegans, etc.|
If you have any questions, please feel free to contact us.
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