CDK1 Gene Editing

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CDK1 Gene Editing    

CDK1 (cyclin-dependent kinase 1), also known as the cell division cycle protein 2 homolog and CDC28, is a key player in cell cycle regulation and a member of the CDK family which is characterized by the need for a separate subunit-cyclin providing the domains necessary for enzymatic activity. Like other cyclin-dependent kinases, CDK1 contains a T-loop that prevents binding of the substrate to the CDK1 active site in the absence of interacting cyclins. There’re several important amino acid side chains in the C-loop leaves, however, the active site is not properly positioned, which makes the orientation of the ATP phosphate to the kinase reaction poor. CDK1 also contains a PSTAIRE helix that moves and rearranges the active site after cyclin binding, thereby promoting CDK1 kinase activity.

The activity of CDK1 is regulated by its binding to the cyclin partner. In addition, CDK1 is regulated by phosphorylation. The phosphorylation of conservative tyrosine (Tyr15 in humans) alters the direction of ATP and prevents potent kinase activity leading to inhibition of CDK1. The Cdk1-cyclin complex is also controlled by the direct binding of CDK inhibitory protein (CKI).

Functionally, CdK1/2 inhibition has the most effective stage of action during S and G 2 and induces E2F transcription factor-dependent cell death. Regulation of CDK1 activity also affects survival checkpoint responses after exposure to DNA damage and microtubule stabilizers. Studies have shown various genetic and epigenetic events lead to widespread over-activity of cell cycle CDKs in human cancers, and their inhibition can lead to cell cycle arrest and apoptosis. Due to the critical role of CDK1 in cell cycle progression and cell transcription, and their relationship to the apoptotic pathway, CDK1 constitutes an attractive target for the development of novel anticancer drugs.

 Regulation of transcriptional programs by Cdk1 during the cell cycleFigure 1: Regulation of transcriptional programs by Cdk1 during the cell cycle (Jorrit M Enserink. 2010)

CDK1 Gene Editing Service

As a global leading biotechnological company specializing in gene editing, CRISPR/Cas9 PlatformCB is dedicated to offering comprehensive CRISPR/Cas9 gene editing services and products to a wide range of genomics researchers. Based on our platform, we can help you effectively control target genes deleted, inserted or point mutated in cells or animals via CRISPR/Cas9 technology.

  • CDK1 Gene Editing Cell Line Generation

We have successfully implemented CDK1 CRISPR/Cas9 gene edited in both easy-to-transfect cell lines and hard-to-transfect cells. With deep gene editing knowledge and extensive experience in experimental operation and data processing, we will offer you full-length custom CDK1 gene editing service from strategy design to final stable cells to support your projects. Our CDK1 gene editing cell line generation services include:

✧ Strategy design
✧ Transfect the cell lines you're interested
✧ Select the high expression cells and sort monoclonal cell
✧ Validate the knockout/knockin/point mutation of CDK1 by PCR and sequencing
✧ Produce cryogenic preserved vials of stable cells and a final report

Typically, we develop CRISPR-mediated gene editing cell lines including HEK239T, Hela, HepG2, U87, but we can use other cell lines according to your requirements.

Other host cell lines available: Ba/F3, CHO, MDA-MB-453, MDA-MB-231NIH3T3, T47D, Neuro2a, MCF7, RKO, K562, RAW264.7, etc.

  • CDK1 Gene Editing Animal Model Generation

CRISPR/Cas9 PlatformCB also has extensive experience in incorporating CRISPR system into animal models, which have been fully recognized by our clients. Tell us your needs, we provide you with custom CDK1 CRISPR/Cas9 gene editing animal service and guarantee at least 2 founders or 3 F1 animals with shorter turnaround time and lower price. Our CDK1 gene editing animal model generation services include:

➢ CDK1 gene conventional knockout animals
➢ CDK1 gene conditional knockout animals
➢ CDK1 point mutation animals
➢ CDK1 knockin animals

Alternative species: mouse, rat, rabbit, zebrafish, C. elegans, etc.

CRISPR/Cas9 PlatformCB is devoted to providing the best gene editing services and products for academic research, biotech research and pharmaceutical drug discovery with excellent quality management and quality assurance capacity. To accelerate the achievement of your research goals, our gene editing expert team provides you with custom CRISPR/Cas9 services to help you solve problems encountered during your research. If you have any question, please feel free to contact us.

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References:

  1. Geoffrey I. Shapiro. et al. Cyclin-Dependent Kinase Pathways As Targets for Cancer Treatment. Clinical Oncology. 2006; 24(11):1770-1783.
  2. Marcos Malumbres. Cyclin-dependent kinases. Genome Biol. 2014; 15(6):122.
  3. Jeffrey PD. et al. Mechanism of CDK activation revealed by the structure of a cyclinA-CDK2 complex. Nature. 1995; 376 (6538):313–320.
  4. Jorrit M Enserink & Richard D Kolodner. An overview of Cdk1-controlled targets and processes Cell Division. 2010; 5:11.
For research use only. Not intended for any clinical use.

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