CA2 Gene Editing

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CA2 Gene Editing    

CA2 (carbonic anhydrase II) is an enzyme responsible for catalyzing the reaction of water and carbon dioxide to form bicarbonate and hydrogen ions. CA2 is a cytoplasmic member of the CA α class and is the most widely distributed isoform of mammalian CA. Defects in CA2 are associated with osteoporosis and renal tubular acidosis. Renal CA reabsorbs bicarbonate ions in the proximal tubules. Loss of carbonic anhydrase activity in bone destroys the ability of osteoclasts to promote bone resorption, leading to osteoporosis. CA2 promotes the transporter activity of monocarboxylate transporters isoforms 1 and 4 (MCT1/4). Increased expression of CA2 is observed in brain development in patients with Alzheimer's disease and Down syndrome. CA2 is also overexpressed in gastrointestinal stromal tumors (GIST) and is considered a useful marker for diagnosis.

Proteins involved in pH regulation in tumors Figure 1: Proteins involved in pH regulation in tumors (Claudiu T. Supuran. 2017)

CA2 Gene Editing Service

CRISPR/Cas9 PlatformCB, a global leading biotechnological company specializing in gene editing, is dedicated to offering comprehensive CRISPR/Cas9 gene editing services and products to a wide range of genomics researchers. With deep gene editing knowledge and extensive experience in experimental operation and data processing, we can help you effectively control target genes deleted, inserted or point mutated in cells or animals via CRISPR/Cas9 technology.

  • CA2 Gene Editing Cell Line Generation

We have successfully implemented CA2 CRISPR/Cas9 gene edited in both easy-to-transfect cell lines and hard-to-transfect cells. To support your projects, we will offer you full-length custom CA2 gene editing service from strategy design to final stable cells. Our CA2 gene editing cell line generation services include:

➢ gRNA design and synthesis
➢ Transfect the cell lines you're interested
➢ Select the high expression cells and sort monoclonal cell
➢ Validate the knockout/knockin/point mutation of CA2 by PCR and sequencing
➢ Produce cryogenic preserved vials of stable cells and a final report

Typically, we develop CRISPR-mediated gene editing cell lines including HEK239T, Hela, HepG2, U87, but we can use other cell lines according to your requirements.

Other host cell lines available: Ba/F3, CHO, MDA-MB-453, MDA-MB-231NIH3T3, T47D, Neuro2a, MCF7, RKO, K562, RAW264.7, etc.

  • CA2 Gene Editing Animal Model Generation

CRISPR/Cas9 PlatformCB also has extensive experience in incorporating CRISPR/Cas9 technology into animal models, which have been fully recognized by our clients. Tell us your needs, we provide a one-stop-shop CA2 CRISPR/Cas9 gene editing animal service and guarantee at least 2 founders or 3 F1 animals with shorter turnaround time and lower price. Our CA2 gene editing animal model generation services include:

➢ CA2 gene conventional knockout animals
➢ CA2 gene conditional knockout animals
➢ CA2 point mutation animals
➢ CA2 knockin animals

Alternative species: mouse, rat, rabbit, zebrafish, C. elegans, etc.

CRISPR/Cas9 PlatformCB is devoted to providing the best gene editing services and products for academic research, biotech research and pharmaceutical drug discovery with excellent quality management and quality assurance capacity. To accelerate the achievement of your research goals, our gene editing expert team provides you with custom CRISPR/Cas9 services for any specific gene to help you solve problems encountered during your research. There is no doubt that CRISPR/Cas9 PlatformCB will be your best partner to support your research.

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References

  1. Palminiello S. et al. Increased levels of carbonic anhydrase II in the developing Down syndrome brain. Brain Res. 2008; 190:193-205.
  2. Parkkila S. et al. Carbonic anhydrase II. A novel biomarker for gastrointestinal stromal tumors. Mod Pathol. 2010; 23(5):743-50.
  3. Claudiu T. Supuran. Carbonic Anhydrases – An Overview. Current Pharmaceutical Design. 2008; 14:603-614 603.
  4. Smith KS. et al. Carbonic anhydrase is an ancient enzyme widespread in prokaryotes. Proc Natl Acad Sci U S A. 1999; 96(26):15184-9.
  5. McMahon C. et al. Bone marrow transplantation corrects osteopetrosis in the carbonic anhydrase II deficiency syndrome. Blood. 2001; 97(7):1947-50.
  6. Claudiu T. Supuran. Carbonic Anhydrase Inhibition and the Management of Hypoxic Tumors. Metabolites. 2017; 7(3):48.
For research use only. Not intended for any clinical use.

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