AHR Gene Editing

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AHR Gene Editing    

AHR (aryl hydrocarbon receptor) is a class of ligand-dependent transcriptional regulators and belongs to the basic helix-loop-helix (bHLH/Per-Arnt-Sim (PAS) protein family (Figure 1). In the absence of a ligand, AHR remains in the cytoplasm and forms a complex with the chaperone proteins HSP90, AIP (XAP2 or ARA9) and p23. Binding of the ligand results in a conformational change that in turn leads to AHR nuclear translocation. AHR releases from its partner and then binds to the bHLH/PAS domain transcription factor-AHR nuclear transporter (ARNT) to form a dimer, the AHR response element (AHRE), to regulate expression of the target gene (e.g. CYP1A1) (Figure 2).

Main domains in the AHR protein Figure 1: Main domains in the AHR protein (Ruggero Ridolf. 2014).

The molecular mechanism of activation of gene expression by the AHR Figure 2: The molecular mechanism of activation of gene expression by the AHR (Michael S. D. 2003).

As a conserved nuclear transcription factor, AHR is expressed in most human cell types, especially in the lung, thymus, kidney, and liver. The main function of AHR is to mediate toxic responses by regulating several metabolic-related enzymes, including cytochrome P450, glutathione S-transferase-α, NAD(P)H reductase-1, and UDP glucose aldehyde, glycosyltransferase. In recent years, AHR research has focused on its effects on inflammation and cancer. However, studies have found that AHR also plays a crucial role in regulating cell proliferation and survival. There is increasing evidence that AHR may regulate several growth factor signaling pathways to induce cell proliferation and survival.

As a conserved nuclear transcription factor, AHR appears to play many roles in cells. Therefore, there is still a need to learn more about the signaling pathways involved in AHR and the more potential functions of AHR.

AHR Gene Editing Service

CRISPR/Cas9 PlatformCB, a global leading biotechnological company specializing in gene editing, is dedicated to offering comprehensive CRISPR/Cas9 gene editing services and products to a wide range of genomics researchers. Based on our platform, we can help you effectively control target genes deleted, inserted or point mutated in cells or animals via CRISPR/Cas9 technology.

  • AHR Gene Editing Cell Line Generation

With deep gene editing knowledge and extensive experience in experimental operation and data processing, we have successfully implemented AHR CRISPR/Cas9 gene edited in both easy-to-transfect cell lines and hard-to-transfect cells. To support your projects, we will offer you full-length custom AHR gene editing service from strategy design to final stable cells. Our AHR gene editing cell line generation services include:

➢ gRNA design and synthesis
➢ Transfect the cell lines you're interested
➢ Select the high expression cells and sort monoclonal cell
➢ Validate the knockout/knockin/point mutation of AHR by PCR and sequencing
➢ Produce cryogenic preserved vials of stable cells and a final report

  • Cell lines we offered includes, but is not limited:

➢ Blood Lineage Cells (RAW264.7, HMC1.2, K562, U937 etc.)
➢ Cancer Cell Lines (HEK293, HEK293T, Hela, MCF7, Neuro2a, HepG2, U87 etc.)
➢ Stem Cells (iPSC)
➢ Other Cell Lines (NIH3T3, MCF10, HEME, SW10 etc.)

  • AHR Gene Editing Animal Model Generation

CRISPR/Cas9 PlatformCB also has extensive experience in incorporating CRISPR/Cas9 technology into animal models, which have been fully recognized by our clients. Tell us your needs, we provide a one-stop-shop AHR CRISPR/Cas9 gene editing animal service and guarantee at least 2 founders or 3 F1 animals with shorter turnaround time and lower price. Our AHR gene editing animal model generation services include:

➢ AHR gene conventional knockout animals
➢ AHR gene conditional knockout animals
➢ AHR point mutation animals
➢ AHR knockin animals

Alternative species: mouse, rat, rabbit, zebrafish, C. elegans, etc.

CRISPR/Cas9 PlatformCB is devoted to providing the best gene editing services and products for academic research, biotech research and pharmaceutical drug discovery with excellent quality management and quality assurance capacity. To accelerate the achievement of your research goals, our gene editing expert team provides you with custom CRISPR/Cas9 services for any specific gene to help you solve problems encountered during your research. There is no doubt that CRISPR/Cas9 PlatformCB will be your best partner to support your research.

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References

  1. Liang Zhou. Ahr function in lymphocytes: emerging concepts. Trends Immunol. 2016 Jan; 37(1): 17–31.
  2. Lucie Larigot. et al. AhR signaling pathways and regulatory functions. Biochim Open. 2018 Dec; 7: 1–9.
  3. Jiuheng Yin. et al. Role of AhR in positive regulation of cell proliferation and survival. Cell Proliferation. 2016; 49(5):554-560.
  4. Patel A. et al. Omeprazole does not potentiate acute oxygen toxicity in fetal human pulmonary microvascular endothelial cells exposed to hyperoxia. Pharm Anal Acta. 2015; 6: 1– 10.
  5. Ruggero Ridolf. et al. AhR (Aryl Hydrocarbon Receptor) Polymorphisms: A Possible Role in TCDD (Dioxins)-AhR Binding and Carcinogenesis. International Journal of Biology. 2014; 6:4.
  6. Michael S. Denison & Scott R. Nagy. ACTIVATION OF THE ARYL HYDROCARBON RECEPTOR BY STRUCTURALLY DIVERSE EXOGENOUS AND ENDOGENOUS CHEMICALS. Annu. Rev. Pharmacol. Toxicol. 2003. 43:309–34.
For research use only. Not intended for any clinical use.

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