ADA Gene Editing

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ADA Gene Editing    

ADA (adenosine deaminase, also known as adenosine aminohydrolase) is a highly conserved purine degrading enzyme that catalyzes adenosine and deoxyadenosine to produce inosine and deoxyinosine (Figure 1). ADA is present in all cells and has the highest expression levels in lymphoid tissues, especially in the thymus, brain, and gastrointestinal tract.

The adenosine deaminase (ADA) metabolism Figure 1: The adenosine deaminase (ADA) metabolism (Aisha Vanessa Sauer. 2012)

Functionally, ADA in the human body is involved in the development and maintenance of the immune system, as well as in epithelial cell differentiation, neurotransmission and pregnancy maintenance. Studies have shown that ADA, in addition to catalyzing adenosine, also stimulates the release of excitatory amino acids and is required for the coupling of A1 adenosine receptors and heterotrimeric G proteins.

Lack of ADA leads to accumulation of t toxic purine degradation by-products that most strongly affect lymphocytes, leading to severe combined immunodeficiency (SCID). Expect the most obvious effect is on lymphocytes, ADA deficient also leads to abnormalities of skeletal, neurodevelopmental, and pulmonary fibrosis. In addition, ADA is used for clinical examination of lymphocytic pleural effusion or peritoneal ascites.

ADA Gene Editing Service

CRISPR/Cas9 PlatformCB, a global leading biotechnological company specializing in gene editing, is dedicated to offering comprehensive CRISPR/Cas9 gene editing services and products to a wide range of genomics researchers. Based on our platform, we can help you effectively control target genes deleted, inserted or point mutated in cells or animals via CRISPR/Cas9 technology.

  • ADA Gene Editing Cell Line Generation

With deep gene editing knowledge and extensive experience in experimental operation and data processing, we have successfully implemented ADA CRISPR/Cas9 gene edited in both easy-to-transfect cell lines and hard-to-transfect cells. To support your projects, we will offer you full-length custom ADA gene editing service from strategy design to final stable cells. Our ADA gene editing cell line generation services include:

➢ Strategy design according to your specific project
➢ gRNA design and synthesis
➢ Transfect the cell lines you're interested
➢ Select the high expression cells and sort monoclonal cell
➢ Validate the knockout/knockin/point mutation of ADA by PCR and sequencing
➢ Produce cryogenic preserved vials of stable cells and a final report

  • Alternative cell lines

➢ Blood Lineage Cells (RAW264.7, HMC1.2, K562, U937 etc.)
➢ Cancer Cell Lines (HEK293, HEK293T, Hela, MCF7, Neuro2a, HepG2, U87 etc.)
➢ Stem Cells (iPSC)
➢ Other Cell Lines (NIH3T3, MCF10, HEME, SW10 etc.)

  • ADA Gene Editing Animal Model Generation

CRISPR/Cas9 PlatformCB also has extensive experience in incorporating CRISPR/Cas9 technology into animal models, which have been fully recognized by our clients. Tell us your needs, we provide a one-stop-shop ADA CRISPR/Cas9 gene editing animal service and guarantee at least 2 founders or 3 F1 animals with shorter turnaround time and lower price. Our ADA gene editing animal model generation services include:

➢ ADA gene conventional knockout animals
➢ ADA gene conditional knockout animals
➢ ADA point mutation animals
➢ ADA knockin animals

Alternative species: mouse, rat, rabbit, zebrafish, C. elegans, etc.

CRISPR/Cas9 PlatformCB is devoted to providing the best gene editing services and products for academic research, biotech research and pharmaceutical drug discovery with excellent quality management and quality assurance capacity. To accelerate the achievement of your research goals, our gene editing expert team provides you with custom CRISPR/Cas9 services for any specific gene to help you solve problems encountered during your research. There is no doubt that CRISPR/Cas9 PlatformCB will be your best partner to support your research. If you have any questions, please feel free to contact us.

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References

  1. H. Bobby Gaspar. et al. How I treat ADA deficiency. Blood. 2009 Oct 22; 114(17): 3524–3532.
  2. Jiménez Castro D. et al. Diagnostic value of adenosine deaminase in nontuberculous lymphocytic pleural effusions. Eur. Respir. J. 2003; 21 (2):220–4.
  3. Aisling M. Flinn & Andrew R. Gennery. Adenosine deaminase deficiency: a review. Orphanet Journal of Rare Diseases. 2018; 13:65.
  4. Aisha Vanessa Sauer. et al. Autoimmune Dysregulation and Purine Metabolism in Adenosine Deaminase Deficiency. Front Immunol. 2012; 3:265.
For research use only. Not intended for any clinical use.

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