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Guaranteed product quality, expert customer support.
CRISPR/Cas9 PlatformCB is a leading biotechnology company focused on gene editing. We can provide solutions for in vivo and in vitro target gene modification and CRISPR system applications, including comprehensive processes such as screening of optimal target sites, guide RNA design and synthesis, vector construction, and quality control.
CRISPR/Cas9 PlatformCB provides you with a CRISPR/Cas9 point mutation rat model construction service. Our team of experienced experts can help you understand what you are investigating and provide you with the most suitable solution.
Strategy of CRISPR/Cas9 point mutation rats
Inject gRNA, Cas9 mRNA and ssDNA into rat fertilized eggs at the same time. ssDNA acts as a DNA repair template, introducing point mutations into target sites during homology-directed repair (HDR).
The workflow of point mutation rat model
Tell us your gene name and point mutations required for knock-in. Our scientists will select the most suitable target sites, maximize the nuclease activity on the target, and minimize off-target reactivity to design the nucleic acid.
Our scientists will optimize your sgRNA sequence to maximize efficiency and minimize off-target effects, construct the nuclease expression vector, and design donor templates and primers for PCR and sequencing-based genotyping analysis.
CRISPR vectors injection to get point mutation knock-in founder rats.
➢ Genotype puppies to identify the founder of knockout genes by PCR and sequencing.
➢ Off-target analysis
➢ Breed the founders to get F1 (optional)
Delivery the correctly edited rats and final report containing all details about experiment program.
The applications of point mutation rats
|For academic research||For biopharmaceuticals research and development|
|Study the functions of specific mutant genes/proteins||Human disease models (including genetic diseases, neurological diseases, cancer, etc.)|
|Analysis of the role of non-coding regions and regulatory factors||Study drug-resistant mutants|
|Screening and verification of disease-causing mutations||Study drug-antibody affinity|
|Study resistant mutations||Pharmacology off-target and efficacy studies,|
|Preclinical safety and toxicity studies|
CRISPR/Cas9 PlatformCB is a pioneer in the frontier of human disease model building. With the help of an experienced team of experts and a professional technology platform, using the CRISPR/Cas9 system, we provide customers with high-quality point mutation rat construction services. CRISPR/Cas9 PlatformCB provides our customers with the most effective and reliable services to best meet their needs and promote the development of the project. If you have any questions or requirements, don't hesitate to contact us.