Knockout Rat Model Service

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Knockout Rat Model Service    

CRISPR/Cas9 PlatformCB provides you with custom rat gene editing services through the CRISPR/Cas9 system and in vitro microinjection platform. We guarantee that at least 2 founders or 3 F1 animals will be eliminated.

What we offered?

  • Constitutive knockout rat
  • Conditional knockout rat (Loxp labeled)

The workflow of CRISPR/Cas9 knockout rat model

Knockout Rat Model Service

  • Step 1. Strategy design
  • ➢ Select most suitable guide RNA target sites
    ➢ Guide RNA design
    ➢ Design donor DNA containing Loxp sequences for conditional knockout rats

  • Step 2. Synthesis & cloning
  • ➢ Synthesize gRNA
    ➢ Synthesize donor DNA
    ➢ Construct CRISPR vector

  • Step 3. Microinjection
  • ➢ gRAN designed for a specific site of the target gene and Cas9 are simultaneously injected into the mouse embryos.
    ➢ Typically, we will inject as many embryos as needed to fulfill the guarantee.

  • Step 4. Validation
  • ➢ Raise the young rats
    ➢ Identification and selection by PCR and sequencing

  • Step 5. Delivery
  • ➢ Delivery the correctly edited rats and final report containing all details about experiment program

Our benefits

  • Two knockout strategies - Constitutive knockout and tissue-specific/inducible knockout
  • More than 3 types of gRNA, optimized gRNA design strategy, maximize efficiency and minimize off-target effects
  • F1 breeding to ensure germline transmission
  • Timely report on project progress
  • Faster turnaround and more economical
  • Convenient and efficient transportation service

Applications of CRISPR/Cas9 rat model

  • Study the biological functions of genes and proteins
  • Building a specific human disease model
  • Drug screening and development
  • Preclinical safety and toxicity studies
  • Verify drug targets and specificities
  • Genomic verification of antibodies

CRISPR/Cas9 PlatformCB has continuously innovative research design, best facilities, and strong quality supervision channels, all of which will help our customers to obtain high-quality results at a lower cost and less time. Optimally designed sgRNA can efficiently target the gene-specific PAM site of your interest and minimize off-target effects. Contact us for further support on CRISPR/Cas9 solutions for in vivo and in vitro applications. There is no doubt CRISPR/Cas9 PlatformCB is the best partner during your program.

For research use only. Not intended for any clinical use.

Inquiry

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