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Knockout Rat Model Service 
CRISPR/Cas9 PlatformCB provides you with custom rat gene editing services through the CRISPR/Cas9 system and in vitro microinjection platform. We guarantee that at least 2 founders or 3 F1 animals will be eliminated.
What we offered?
- Constitutive knockout rat
- Conditional knockout rat (Loxp labeled)
The workflow of CRISPR/Cas9 knockout rat model
- Step 1. Strategy design
- Step 2. Synthesis & cloning
- Step 3. Microinjection
- Step 4. Validation
- Step 5. Delivery
➢ Select most suitable guide RNA target sites
➢ Guide RNA design
➢ Design donor DNA containing Loxp sequences for conditional knockout rats
➢ Synthesize gRNA
➢ Synthesize donor DNA
➢ Construct CRISPR vector
➢ gRAN designed for a specific site of the target gene and Cas9 are simultaneously injected into the mouse embryos.
➢ Typically, we will inject as many embryos as needed to fulfill the guarantee.
➢ Raise the young rats
➢ Identification and selection by PCR and sequencing
➢ Delivery the correctly edited rats and final report containing all details about experiment program
Our benefits
- Two knockout strategies - Constitutive knockout and tissue-specific/inducible knockout
- More than 3 types of gRNA, optimized gRNA design strategy, maximize efficiency and minimize off-target effects
- F1 breeding to ensure germline transmission
- Timely report on project progress
- Faster turnaround and more economical
- Convenient and efficient transportation service
Applications of CRISPR/Cas9 rat model
- Study the biological functions of genes and proteins
- Building a specific human disease model
- Drug screening and development
- Preclinical safety and toxicity studies
- Verify drug targets and specificities
- Genomic verification of antibodies
CRISPR/Cas9 PlatformCB has continuously innovative research design, best facilities, and strong quality supervision channels, all of which will help our customers to obtain high-quality results at a lower cost and less time. Optimally designed sgRNA can efficiently target the gene-specific PAM site of your interest and minimize off-target effects. Contact us for further support on CRISPR/Cas9 solutions for in vivo and in vitro applications. There is no doubt CRISPR/Cas9 PlatformCB is the best partner during your program.