Knock-in Rats Model    

CRISPR/Cas9 Platform^CB offers you CRISPR-based gene knock-in rats at very competitive prices. those models are suitable for a variety of applications, including reporter gene expression, conditional gene expression, and disease models. We guarantee at least 2 founders or 3 F1 animals. Tell us the name of your gene and the target site you want to target; we will generate your rat model.

Our knock-in rats model includes

• Fluorescent proteins or immune-tags insertion, custom tags and combinations are also available.
• Cre gene and Floxed allele insertion (for conditionally knockout target gene).
• Large fragment insertion at Rosa26 - Targeting Rosa26 can control gene copy number and gene expression, and avoid silencing or suppressing the expression of neighboring genes.

Project workflow

• Step1. Knock-in strategy design

Consult our team of experts and tell us what genes and loci you want to knock in. We will customize high-efficiency targeting strategies for you, including the most suitable PAM sites, donor DNA and efficient gRNA sequences design, as well as genotyping primers design based on PCR and sequencing.

• Step2. Knock-in vector construction

Synthesize gRNA, Cas9 mRNA, donor DNA, and construct CRISPR/Cas9 vector.

• Step3. CRISPR/Cas9 injection

gRNA, Cas9 mRNA and donor DNA are simultaneously implanted into rat single-cell embryos (as many as possible).

• Step4. Founder screening

Select the young rats with right gene editing by PCR and sequencing.

• Step5. Breeding founders to obtain F1 (optional)
• Step6. Delivery

We will delivery at least 2 founders or 3 F1 rats, and final report which details each step, including target vector design, construction and validation, transfection conditions, genotyping strategies and results.

Applications of knock-in rats

For academic research	For biopharmaceuticals research and development
Study the functions of specific genes/proteins	Human disease models (including genetic diseases, neurological diseases, cancer, etc.)
Study disease-causing transgene	Selectivity screening
Quantitative assessment of gene expression and protein localization	Drug screening and development
Study the role of transcriptional regulatory elements

Our features and benefits

• New CRISPR strategy improves efficiency and success rate
• F1 breeding to ensure germline transmission
• Report project progress in a timely manner
• Fast turnaround

CRISPR/Cas9 Platform^CB is a leading biotechnology company focused on gene editing. We have extensive experience in processing animal embryos and have been very successful in integrating CRISPR/Cas9 technology into our procedures, which has been well received by customers. From the early strategic design to the final model, we guarantee to provide our customers with excellent service. Tell us the genes you are interested in and we will meet your specific requirements and protect your scientific investment.