CMV-SV40/LTA(GFP, Puro) Lentiviral Particles

The CMV-SV40/LTA(GFP, Puro) lentiviral particle is a sophisticated tool for cell biology research, designed to efficiently deliver the SV40 large T antigen (LTA) gene to target cells. This lentivirus utilizes a cytomegalovirus (CMV) promoter to drive high-level expression of SV40 LTA. SV40 LTA is a potent oncoprotein that immortalizes primary cells by inhibiting tumor suppressor pathways. The vector also integrates a GFP reporter gene for real-time observation of transduced cells under a fluorescence microscope, and a puromycin resistance gene for screening stable cell populations. This dual-screening system ensures high transduction efficiency and simplifies the isolation of genetically modified cells. The lentiviral backbone further enhances safety and versatility, enabling stable integration into both dividing and non-dividing cells without triggering significant immune responses. These properties make it an ideal vector for long-term studies requiring sustained transgenic expression.

This lentiviral vector is primarily used for cell immortalization, a crucial process for extending the lifespan of primary cells (such as fibroblasts, epithelial cells, or stem cells) that age after a limited number of passages. By introducing SV40 LTA, researchers can establish cell lines that proliferate sustainably and retain key physiological characteristics, thereby facilitating long-term experiments in fields such as drug screening, cancer biology, and regenerative medicine. Other applications include building stable cell lines for protein production, studying viral carcinogenesis mechanisms, and developing cell models of aging or neurodegenerative diseases. In summary, this lentivirus provides a powerful and versatile platform for advancing cell and molecular research.