CMV-SV40/LTA(Bla) Lentiviral Particles

CMV-SV40/LTA (Bla) lentiviral particles are sophisticated tools for gene manipulation and cell engineering, designed to efficiently deliver the SV40 large T antigen (LTA) gene to target cells. This lentiviral vector system utilizes a robust cytomegalovirus (CMV) promoter, ensuring stable expression of the SV40 LTA transgene in a variety of mammalian cell types. The introduced blast fungicide resistance gene (Bla) provides an effective screening mechanism, enabling researchers to enrich stably transduced cells under blast fungicide treatment. HIV-1-derived lentiviral vectors offer numerous inherent advantages, including the ability to transduce both dividing and non-dividing cells, long-term transgene expression through genome integration, and good safety due to their self-inactivation (SIN) design. The high titers achievable with these particles further enhance their application value, enabling efficient gene delivery even in difficult-to-transduce cell types such as primary cells or stem cells.

The primary application of these lentiviral particles is in cell immortalization, a key technology for extending the lifespan of primary cells that otherwise age after a limited number of passages in vitro. The SV40 large T antigen is a potent oncoprotein that inactivates key tumor suppressor proteins such as p53 and retinoblastoma protein (pRb), thereby bypassing cellular senescence checkpoints and achieving unlimited proliferation. This immortalization capability is crucial for constructing stable cell lines from tissues that are difficult to culture long-term, such as human epithelial cells, fibroblasts, and specialized cell types like hepatocytes or neurons. These immortalized cells retain many physiological characteristics of their primary cells, making them ideal models for studying disease mechanisms, drug screening, toxicological assessments, and molecular signaling pathways. In addition to immortalization, lentiviral particles can also be used in functional genomics research to explore the role of SV40 LTA in cell transformation, DNA replication, and viral pathogenesis.