EF1a-hKRAS/G12V(GFP, Puro) Lentiviral Particles

The EF1a-hKRAS/G12V (GFP, Puro) Lentiviral Particles provide a highly efficient system for the stable delivery and expression of the oncogenic human KRAS G12V mutation in mammalian cells. Driven by the constitutive EF1a promoter, the vector ensures high-level expression across various cell lines, including those typically resistant to traditional transfection or prone to promoter silencing, such as primary and stem cells. This specific KRAS variant contains a substitution at codon 12, resulting in a constitutively active GTP-bound state that drives uncontrolled cellular signaling. The particles are engineered as third-generation, replication-deficient units, incorporating a bicistronic expression cassette that includes Green Fluorescent Protein (GFP) for direct visualization and a Puromycin resistance marker for the selection of stable clones. This integration facilitates both the immediate assessment of transduction efficiency and the long-term maintenance of the transgene.

These lentiviral particles are indispensable tools for investigating the molecular drivers of oncogenesis and evaluating targeted therapeutic interventions. Researchers utilize them to establish stable cell lines that mimic the signaling environment of KRAS-driven cancers, such as pancreatic, colorectal, and lung adenocarcinomas. Beyond basic signaling research into the MAPK and PI3K pathways, these particles are extensively used in high-throughput drug screening to identify small molecules that specifically inhibit mutant KRAS or its downstream effectors. The GFP reporter enables sophisticated applications, including longitudinal live-cell imaging, flow cytometry-based population analysis, and the tracking of tumor cell migration or invasion in 3D organoid models. Furthermore, the ability to generate stable, antibiotic-selected populations makes these particles ideal for in vivo studies, including subcutaneous or orthotopic xenograft models, where consistent expression of the G12V mutant is required to observe phenotypic changes in tumor growth and metastasis.