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E.coli Genome Editing Service 
E. coli is widely used in industrial microbial production. Using metabolic engineering and synthetic biology methods to optimize the expression and regulation of microbial genes on the genome can increase the production of chemicals, bioenergy products, and recombinant proteins. With the breakthrough of CRISPR/Cas9 technology, the genome modification of bacteria has become more and more efficient and accurate.
CRISPR/Cas9 PlatformCB launches E. coli genome transformation service for gene knock-in, knock-out or gene replacement in E. coli. In order to further improve the efficiency of gene editing in E. coli, CRISPR/Cas9 PlatformCB has developed a novel λRed-CRISPR/Cas9 technology. This system uses the efficiency of traditional λRed recombination and the simplicity of CRISPR/Cas9 for seamless and targeted genome editing.
Our E. coli genome editing service details include, but not limited to
- CRISPR/Cas9 gene knockout - Some or all of your selected genes are missing
- CRISPR/Cas9 gene knock-in - Mark existing genes or knock-in genes of interest
- Proven safe harbor loci for stable expression of genes or constructs of interest.
➢ sgRNA vector design/construction (more than 3 types of sgRNA).
Our scientists will screen for the most suitable PAM sites and optimize your sgRNA sequence to maximize efficiency and minimize off-target effects.
➢ Gene knockout
➢ Clonal screening and selection
➢ Verify genome editing by PCR and sequencing
➢ sgRNA vector design/construction (more than 3 types of sgRNA) and donor DNA construction.
Our scientists will screen for the most suitable PAM site and optimize your sgRNA sequence to maximize efficiency and minimize off-target effects.
➢ Gene knock-in
➢ Clonal screening and selection
➢ Verify genome editing by PCR and sequencing
➢ sgRNA vector design/construction (more than 3 types of sgRNA) and donor DNA construction.
Our scientists will screen the most suitable PAM site and optimize your sgRNA sequence to maximize efficiency and minimize off-target effects.
➢ Gene knock-in at safe harbor location
➢ Clonal screening and selection
➢ Verify genome editing by PCR and sequencing
Strategy of E. coli λRed-CRISPR/Cas9 genome editing
Advantages of CRISPR/Cas9 PlatformCB E. coli gene editing systems
- Seamless
- Multi-gene editing - Up to 3 genes can be eliminated at the same time
- Accurate to base pairs
- Easy to select: no optional markup required
CRISPR/Cas9 PlatformCB is a leading biotechnology company specializing in providing a wide range of gene editing services and products. Our high-quality data and the efficiency of the experimental cycle meet the needs from early sgRNA design and synthesis to model organism construction and have been well received by customers. We guarantee our clients the most reliable and efficient research services to best match their research goals.