EF1a-SV40/LTA(GFP, Puro) Lentiviral Particles

The EF1a-SV40/LTA (GFP, Puro) Lentiviral Particles are high-titer, replication-deficient viral vectors designed for the stable delivery and constitutive expression of the SV40 Large T Antigen (LTA). This construct is driven by the human elongation factor-1 alpha (EF1a) promoter, which is known for its high-level expression and resistance to gene silencing, making it particularly effective in primary cells, stem cells, and other challenging mammalian cell types. To facilitate experimental tracking and selection, the particles incorporate a Green Fluorescent Protein (GFP) reporter for the visual assessment of transduction efficiency and a Puromycin resistance marker for the generation of stable cell lines. Produced using a third-generation lentiviral packaging system, these particles ensure high biosafety standards and robust integration into the host genome, providing a reliable platform for permanent genetic modification.

The primary application of these lentiviral particles is the immortalization of primary cells to establish continuous cell lines. By sequestering key tumor suppressor proteins, specifically p53 and the retinoblastoma (pRb) family, the SV40 Large T Antigen allows cells to bypass replicative senescence and maintain a proliferative state. This makes it an essential tool for researchers who need to expand limited primary cell populations for long-term functional studies. Beyond immortalization, these particles are widely used in research concerning cell cycle regulation, oncogenic transformation, and viral DNA replication mechanisms. The integrated GFP and Puromycin markers streamline the experimental workflow, allowing for the rapid identification and selection of successfully transduced populations. Whether used for developing robust cellular models for drug screening or investigating the molecular pathways of cancer progression, these lentiviral particles offer a high-performance solution for long-term transgene expression.