STAT1 Knockout Cell Line-MDCKII
Cat.No. : CSC-RT2797
Host Cell: MDCKII Target Gene: STAT1
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
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Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT2797 |
| Introduction | This cell line has a good performance in the viral vaccine (e.g. influenza virus) production and could significantly increase your viral caccine production capacity. |
| Cell Line Information | This cell is a stable cell line with a homozygous knockout of STAT1. |
| Target Gene | STAT1 |
| Host Cell | MDCKII |
| Applications | This cell line has a good performance in the viral vaccine (e.g. influenza virus) production and could significantly increase your viral caccine production capacity. |
| Size Form | 1 vial (>10^6 cell/vial) |
| Shipping | Dry ice package |
| Storage | Liquid Nitrogen |
| Species | Canis lupus familiaris (Dog) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Background
Applications
The STAT1 (signal transducer and activator of transcription 1) gene encodes a transcription factor that is essential for a variety of cellular processes, including cell survival, proliferation, and pathogen response. STAT1 is part of the STAT family of proteins and upon activation is phosphorylated by receptor-associated kinases. This phosphorylation triggers dimerization (forming homo- or heterodimers) and promotes the translocation of STAT1 to the nucleus, where it functions as a transcription factor. STAT1 activation can be stimulated by a variety of ligands, including interferon alpha (IFNα), interferon gamma (IFNγ), epidermal growth factor (EGF), platelet-derived growth factor (PDGF), interleukin 6 (IL-6), and IL-27. Notably, type I interferons (IFN-α, IFN-β) interact with their receptors and initiate a signaling cascade through the kinases JAK1 and TYK2, which phosphorylate and activate STAT1 and STAT2. Activated STAT molecules form dimers and bind to the ISGF3G/IRF-9 complex, allowing STAT1 to enter the nucleus and regulate gene expression.
STAT1 gene mutations can result in gain of function (GOF) or loss of function (LOF), resulting in different phenotypic outcomes. Both mutation types are associated with increased susceptibility to infection. GOF mutations are commonly seen in patients with chronic mucocutaneous candidiasis (CMC), leading to persistent Candida infections and increased autoimmune and other infectious diseases. In contrast, LOF mutations can result in partial or complete deficiency of STAT1, impaired responses to IFN-α, IFN-β, and IL-27, making individuals more susceptible to bacterial, viral, and fungal infections.
1. Viral Infection Studies: The Signal Transducer and Activator of Transcription 1 (STAT1) plays a critical role in mediating the response to viral infections. Using MDCKII cells with a knockout of the STAT1 gene allows researchers to study viral pathogenesis and host-virus interactions in the absence of a functional STAT1 pathway.
2. Cancer Research: STAT1 has dual roles in cancer, acting as both a tumor suppressor and, in some contexts, as a promoter of tumorigenesis. The STAT1 Knockout MDCKII Cell Line can be employed to elucidate the molecular mechanisms behind STAT1’s involvement in different cancer types. Researchers can analyze how the absence of STAT1 affects cell proliferation, apoptosis, and metastasis, thereby identifying potential therapeutic targets.
3. Immune Response Studies: STAT1 is a pivotal component of the immune signaling pathways, particularly in the type I and type II interferon responses. The STAT1 Knockout MDCKII Cell Line can be utilized to study the specific contributions of STAT1 in immune responses.
4. Drug Screening and Development: This cell line provides a unique model for high-throughput screening of antiviral and anticancer compounds. By examining the efficacy and mechanism of action of new drug candidates in STAT1-deficient conditions, researchers can better predict their potential effectiveness and toxicity in vivo, especially concerning STAT1-related pathways.
5. Inflammatory Disease Research: STAT1 is involved in the regulation of genes associated with inflammation. The STAT1 Knockout MDCKII Cell Line can model inflammatory diseases to study the downstream effects of STAT1 deficiency on inflammatory signaling molecules, cytokine production, and immune cell behavior.
For research use only. Not intended for any clinical use.
