LMAN2 Easy KO Kit
Cat.No.
CC-762
Description
A complete kit for efficient gene knockout in mammalian cells, combining chemically synthesized sgRNAs with Cas9 RNPs to induce targeted DNA cleavage and generate frameshift mutations or deletions. All essential reagents for transfection and knockout validation are included for rapid, high-efficiency gene disruption.
Applications
This kit enables in vitro gene knockout in human-derived cells using chemically synthesized sgRNAs and Cas9-gRNA RNP complexes. Transfected RNPs cleave early exons of the target gene, inducing deletions or frameshift mutations for efficient and rapid knockout.
Features
- All-in-One workflow from gene editing to knockout validation for users with no prior experience.
- Pre-validated sgRNAs and primers for rapid setup.
- Streamlined experiment handling.
- CRISPR RNP method ensures precise and efficient gene knockout.
Species
Human
Classification
Gene-specific KO Kit
NCBI Gene ID
10960
Ensembl ID
ENSG00000169223
UniProt ID
Q12907
GeneAbbr
LMAN2 C5orf8
Reactions
5–10 reactions per target gene
Kit Components
2–3 chemically synthesized sgRNAs (200pmol each)
3 PCR/Sequencing primers (500pmol each)
LM cell lysate (500µL)
Cas9 protein (12µg)
LM RNP transfection reagent (50µL)
3 PCR/Sequencing primers (500pmol each)
LM cell lysate (500µL)
Cas9 protein (12µg)
LM RNP transfection reagent (50µL)
Storage Conditions
Store at -80°C for up to 1 year or at -20°C for up to 6 months. Avoid repeated freeze-thaw cycles.
For research use only. Not intended for any clinical use.
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