FLT4 Easy KO Kit
Cat.No.
CC-504
Description
A complete kit for efficient gene knockout in mammalian cells, combining chemically synthesized sgRNAs with Cas9 RNPs to induce targeted DNA cleavage and generate frameshift mutations or deletions. All essential reagents for transfection and knockout validation are included for rapid, high-efficiency gene disruption.
Applications
This kit enables in vitro gene knockout in human-derived cells using chemically synthesized sgRNAs and Cas9-gRNA RNP complexes. Transfected RNPs cleave early exons of the target gene, inducing deletions or frameshift mutations for efficient and rapid knockout.
Features
- All-in-One workflow from gene editing to knockout validation for users with no prior experience.
- Pre-validated sgRNAs and primers for rapid setup.
- Streamlined experiment handling.
- CRISPR RNP method ensures precise and efficient gene knockout.
Species
Human
Classification
Gene-specific KO Kit
NCBI Gene ID
2324
Ensembl ID
ENSG00000037280
UniProt ID
P35916
GeneAbbr
FLT4 VEGFR3
Reactions
5–10 reactions per target gene
Kit Components
2–3 chemically synthesized sgRNAs (200pmol each)
3 PCR/Sequencing primers (500pmol each)
LM cell lysate (500µL)
Cas9 protein (12µg)
LM RNP transfection reagent (50µL)
3 PCR/Sequencing primers (500pmol each)
LM cell lysate (500µL)
Cas9 protein (12µg)
LM RNP transfection reagent (50µL)
Storage Conditions
Store at -80°C for up to 1 year or at -20°C for up to 6 months. Avoid repeated freeze-thaw cycles.
For research use only. Not intended for any clinical use.
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