DAPK1 Knockout Cell Line-HEK293T
Cat.No. : CSC-RT1765
Host Cell: HEK293T Target Gene: DAPK1
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
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Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT1765 |
| Cell Line Information | HEK293T -DAPK1(-/-) is a stable cell line with a homozygous knockout of human DAPK1 using CRISPR/Cas9. |
| Target Gene | DAPK1 |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Shipping | 1 vial of knockout cell line |
| Storage | Liquid nitrogen |
| Gene Symbol | DAPK1 |
| Gene ID | 1612 |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Background
Applications
Death-associated protein kinase 1 (DAPK1) is a serine/threonine kinase that plays a crucial role in multiple cellular processes, including apoptosis, autophagy, and cytoskeletal dynamics. The protein is encoded by the DAPK1 gene located on chromosome 9q21.33 and is characterized by an N-terminal kinase domain followed by a calmodulin-binding domain, ankyrin repeats, and a cytoskeletal binding region. As a pro-apoptotic kinase, DAPK1 is activated by various stimuli, such as cytokines, endoplasmic reticulum stress, and oxidative stress, leading to the phosphorylation of downstream substrates that promote apoptosis. For example, DAPK1 can phosphorylate and activate the well-known tumor suppressor protein p53, thereby enhancing the apoptotic response.
In addition to apoptosis, DAPK1 is also involved in the regulation of autophagy. DAPK1 can activate autophagy by preventing the binding of the autophagy inhibitory complex Beclin-1/Bcl-2, thereby promoting cell survival under nutrient-deficient conditions. This dual role in promoting cell death and survival emphasizes the importance of this protein in balancing cellular outcomes based on environmental cues. In addition, DAPK1 affects cytoskeletal organization and cell motility. It does this by interacting with microtubule-associated proteins and mediating cytoskeletal rearrangements. This property is particularly important in processes such as cancer metastasis, where cell motility and invasion play a key role.
Death-associated protein kinase 1 (DAPK1) is a serine/threonine kinase that has been implicated in a variety of cellular processes, including apoptosis, autophagy, and cytoskeletal organization. The creation of a DAPK1 knockout (KO) cell line using HEK293T cells provides a valuable tool for studying the biological role of DAPK1 and its potential impact in disease. Here are some potential applications for DAPK1 knockout cell lines-HEK293T:
Cancer research: DAPK1 is generally considered a tumor suppressor. DAPK1 KO HEK293T cells can be used to study how loss of DAPK1 leads to tumorigenic processes, such as uncontrolled cell proliferation, resistance to apoptosis, and increased metastatic potential.
Drug screening: Screen for novel compounds or drugs that can compensate for the loss of DAPK1 function or modulate its pathways. This cell line can serve as a model for identifying potential therapeutic agents.
Signal transduction studies: Understand the signaling pathways regulated by DAPK1. By examining DAPK1 knockout HEK293T cells, researchers can dissect downstream effects and interactions within cellular signaling networks.
Gene function analysis: Elucidate the specific function of DAPK1 by comparing the phenotypic differences between wild-type and knockout cell lines. This is critical for understanding its role in cellular processes such as apoptosis, autophagy, and cytoskeletal reorganization.
Disease modeling: Create models for diseases associated with DAPK1, such as neurodegenerative and autoimmune diseases. DAPK1 knockout cell lines provide insights into disease mechanisms and potential interventions.
For research use only. Not intended for any clinical use.
