Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RI0051
Host Cell : HEK293 Size : >1x106 frozen cells/vial
| Cat. No. | CSC-RI0051 |
| Description | This cell line is engineered to overexpress human SCN5A. |
| Background | Nav1.5 is expressed in cardiac myocytes and involved with action potential initiation and conduction. Mutations of Nav1.5 result in QT prolongation and ventricular fibrillation. This ion channel is a target for anti-arrhythmic drugs and the toxic side effects of local anaesthetics. |
| Target Gene | SCN5A |
| Gene Species | Homo sapiens (Human) |
| Abbr | HEK293-HuSCN5A |
| Alias | SCN5A, CMD1E, CDCD2, CMPD2, HB1, HB2, HBBD, HH1, ICCD, IVF, LQT3, Nav1.5, PFHB1, SSS1, VF1, CMD1E |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications |
1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research |
| Size | >1x106 frozen cells/vial |
| Stability | Validated for at least 10 passages |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Storage | Liquid nitrogen |
| Shipping | Dry ice |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Morphology | Epithelial |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
| Gene Name | SCN5A sodium channel, voltage-gated, type V, alpha subunit [ Homo sapiens ] |
| Gene Symbol | SCN5A |
| Synonyms | HB1; HB2; HH1; IVF; VF1; HBBD; ICCD; LQT3; SSS1; CDCD2; CMD1E; CMPD2; PFHB1; Nav1.5 |
| Gene Description | sodium channel, voltage-gated, type V, alpha subunit |
| GeneID | 6331 |
| Uni ProtID | Q14524 |
| mRNA Refseq | NM_000335.4 |
| Protein Refseq | NP_000326.2 |
| Chromosome Location | 3p21 |
| Function | ankyrin binding; calmodulin binding; ion channel binding; protein binding; scaffold protein binding; voltage-gated sodium channel activity; voltage-gated sodium channel activity involved in regulation of cardiac muscle cell action potential; voltage-gated sodium channel activity involved in regulation of cardiac muscle cell action potential; |
| Pathway | Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Interaction between L1 and Ankyrins, organism-specific biosystem; L1CAM interactions, organism-specific biosystem; SIDS Susceptibility Pathways, organism-specific biosystem; |
| MIM | 600163 |
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This SCN5A HEK293 cell line is an absolute must-have for cardiac safety pharmacology. We get robust, reproducible sodium currents in our automated patch-clamp system. Highly recommended for its excellent viability and stable expression.
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