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Human SCN5A Stable Cell Line-HEK293

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RI0051

Host Cell :   HEK293 Size :   >1x106 frozen cells/vial

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Cell Line Information

Cell Culture Information

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Gene Information

Documents

Cat. No. CSC-RI0051
Description This cell line is engineered to overexpress human SCN5A.
Background Nav1.5 is expressed in cardiac myocytes and involved with action potential initiation and conduction. Mutations of Nav1.5 result in QT prolongation and ventricular fibrillation. This ion channel is a target for anti-arrhythmic drugs and the toxic side effects of local anaesthetics.
Target Gene SCN5A
Gene Species Homo sapiens (Human)
Abbr HEK293-HuSCN5A
Alias SCN5A, CMD1E, CDCD2, CMPD2, HB1, HB2, HBBD, HH1, ICCD, IVF, LQT3, Nav1.5, PFHB1, SSS1, VF1, CMD1E
Host Cell HEK293
Host Cell Species Homo sapiens (Human)
Applications

1. Gene expression studies

2. Signaling pathway research

3. Drug screening and toxicology

4. Channelopathies research

Size >1x106 frozen cells/vial
Stability Validated for at least 10 passages
Quality Control Negative for bacteria, yeast, fungi and mycoplasma.
Storage Liquid nitrogen
Shipping Dry ice
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium Complete medium supplemented with 10% (v/v) DMSO
Morphology Epithelial
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name SCN5A sodium channel, voltage-gated, type V, alpha subunit [ Homo sapiens ]
Gene Symbol SCN5A
Synonyms HB1; HB2; HH1; IVF; VF1; HBBD; ICCD; LQT3; SSS1; CDCD2; CMD1E; CMPD2; PFHB1; Nav1.5
Gene Description sodium channel, voltage-gated, type V, alpha subunit
GeneID 6331
Uni ProtID Q14524
mRNA Refseq NM_000335.4
Protein Refseq NP_000326.2
Chromosome Location 3p21
Function ankyrin binding; calmodulin binding; ion channel binding; protein binding; scaffold protein binding; voltage-gated sodium channel activity; voltage-gated sodium channel activity involved in regulation of cardiac muscle cell action potential; voltage-gated sodium channel activity involved in regulation of cardiac muscle cell action potential;
Pathway Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Interaction between L1 and Ankyrins, organism-specific biosystem; L1CAM interactions, organism-specific biosystem; SIDS Susceptibility Pathways, organism-specific biosystem;
MIM 600163
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Reliable Currents for Patch-Clamp Studies

This SCN5A HEK293 cell line is an absolute must-have for cardiac safety pharmacology. We get robust, reproducible sodium currents in our automated patch-clamp system. Highly recommended for its excellent viability and stable expression.

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