Human SCN2A Stable Cell Line-HEK293

Name: Human SCN2A Stable Cell Line-HEK293
SKU: CSC-RI0157
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0157

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0157
Description	This cell line is engineered to overexpress human SCN2A.
Background	The human SCN2A gene encodes the pore-forming subunit of Nav1.2, a voltage-gated sodium channel expressed in CNS neurons. SCN2A mutations are associated with benign familial neonatal-infantile seizures. Nav1.2 channels are therapeutic targets in seizure, stroke, and pain.
Target Gene	SCN2A
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuSCN2A
Alias	SCN2A, BFIC3, BFIS3, BFNIS, EIEE11, HBA, HBSCI, NAC2, Na(v)1.2, Nav1.2, SCN2A1, SCN2A2
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	SCN2A sodium channel, voltage-gated, type II, alpha subunit [ Homo sapiens ]
Gene Symbol	SCN2A
Synonyms	HBA; NAC2; BFIC3; BFIS3; BFNIS; HBSCI; EIEE11; HBSCII; Nav1.2; SCN2A1; SCN2A2; Na(v)1.2
Gene Description	sodium channel, voltage-gated, type II, alpha subunit
Gene ID	6326
Uni Prot ID	Q99250
m RNA Refseq	NM_001040143.1
Protein Refseq	NP_001035233.1
Chromosome Location	2q24.3
Function	voltage-gated sodium channel activity;
Pathway	Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Interaction between L1 and Ankyrins, organism-specific biosystem; L1CAM interactions, organism-specific biosystem;
MIM	182390

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