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Human SCN11A Stable Cell Line - HEK293

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RI0206

Host Cell :   HEK293 Size :   >1x106 frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RI0206
Description This cell line is engineered to stably overexpress Human SCN11A in HEK293 cells.
Target Gene SCN11A
Gene Species Homo sapiens (Human)
Host Cell HEK293
Host Cell Species Homo sapiens (Human)
Applications

1. Gene expression studies

2. Signaling pathway research

3. Drug screening and toxicology

4. Channelopathies research

Size >1x106 frozen cells/vial
Stability Validated for at least 10 passages
Quality Control Negative for bacteria, yeast, fungi and mycoplasma.
Storage Liquid nitrogen
Shipping Dry ice
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium Complete medium supplemented with 10% (v/v) DMSO
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name SCN11A sodium channel, voltage-gated, type XI, alpha subunit [ Homo sapiens ]
Gene Symbol SCN11A
Synonyms NaN; SNS-2; NAV1.9; SCN12A
Gene Description sodium channel, voltage-gated, type XI, alpha subunit
Gene ID 11280
Uni Prot ID Q9UI33
m RNA Refseq NM_014139.2
Protein Refseq NP_054858.2
Chromosome Location 3p22.2
Function voltage-gated sodium channel activity;
MIM 604385
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Customer Q&As
What type of cells does Human SCN11A Stable Cell Line - HEK293 belong to?

A: Epithelial cells.

What type of culture medium should be used for this cell line?

A: DMEM.

Can Human SCN11A Stable Cell Line - HEK293 be cultured in serum-free conditions?

A: Can be cultured for a short period.

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