Human SCN9A Stable Cell Line-HEK293

Name: Human SCN9A Stable Cell Line-HEK293
SKU: CSC-RI0053
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0053

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0053
Description	This cell line is engineered to overexpress human SCN9A.
Target Gene	SCN9A
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuSCN9A
Alias	SCN9A, ETHA, Nav1.7, NE NA, NENA, PN1, hNE-Na, SFNP, FEB3B, NE-NA, GEFSP7
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	SCN9A sodium channel, voltage-gated, type IX, alpha subunit [ Homo sapiens ]
Gene Symbol	SCN9A
Synonyms	PN1; ETHA; NENA; SFNP; FEB3B; NE-NA; GEFSP7; Nav1.7
GeneID	6335
Uni ProtID	Q15858
mRNA Refseq	NM_002977.3
Protein Refseq	NP_002968.1
Chromosome Location	2q24
Function	sodium ion binding; voltage-gated sodium channel activity;
Pathway	Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Interaction between L1 and Ankyrins, organism-specific biosystem; L1CAM interactions, organism-specific biosystem;
MIM	603415

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Nav1.7 is a voltage-gated sodium channel alpha subunit. It is expressed in all types of DRG neurons, sympathetic neurons, Schwann cells and neuroendocrine cells. This channel is involved in action potential initiation and transmission in peripheral neurons and is a potential target for local anaesthetic agents. Specific hereditary mutations in the gene encoding Nav1.7, SCN9A, result in the painful neuropathies, erythermalgia and paroxysmal extreme pain disorder, that can be ascribed to altered firing of pain sensing neurons. Changes in the expression levels of the channel correlates with increases in neuronal excitability in acute and chronic pain syndromes. Hence due to their relatively discrete location and fundamental role in mediating certain types of pain they may be promising targets for pharmacological intervention.

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