Human SCN4A Stable Cell Line-HEK293

Name: Human SCN4A Stable Cell Line-HEK293
SKU: CSC-RI0050
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0050

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0050
Description	This cell line is engineered to overexpress human SCN4A.
Background	Nav1.4 is primarily expressed in skeletal muscle and involved in the initiation and transmission of muscle action potentials. This ion channel is a target for local anaesthetics used in the treatment of myotonia, a disorder characterized by the slow relaxation of muscles after voluntary contraction or electrical stimulation. Myotonia is a symptom commonly seen in patients with myotonic muscular dystrophy.
Target Gene	SCN4A
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuSCN4A
Alias	SCN4A, HOKPP2, HYKPP, HYPP, NAC1A, Na(V)1.4, Nav1.4, SkM1
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	SCN4A sodium channel, voltage-gated, type IV, alpha subunit [ Homo sapiens ]
Gene Symbol	SCN4A
Synonyms	HYPP; SkM1; HYKPP; NAC1A; HOKPP2; Nav1.4; Na(V)1.4
Gene Description	sodium channel, voltage-gated, type IV, alpha subunit
GeneID	6329
Uni ProtID	P35499
mRNA Refseq	NM_000334.4
Protein Refseq	NP_000325.4
Chromosome Location	17q23.3
Function	voltage-gated sodium channel activity;
Pathway	Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Interaction between L1 and Ankyrins, organism-specific biosystem; L1CAM interactions, organism-specific biosystem;
MIM	603967

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