Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RI0164
Host Cell : CHO-K1 Size : >1x106 frozen cells/vial
| Cat. No. | CSC-RI0164 |
| Description | This cell line is engineered to overexpress human SCN5A. |
| Background | The human SCN5A gene encodes the pore-forming subunit of Nav1.5, a voltage-gated sodium channel expressed in heart and upregulated in breast cancer cells. Mutations in SCN5A are responsible for some forms of long QT and short QT syndromes, progressive cardiac conduction defect, sick sinus node syndrome, and dilated cardiomyopathy, Brugada syndrome, congenital heart block, Nav1.5 channels are anti-targets in cardiac risk assessment. |
| Target Gene | SCN5A |
| Gene Species | Homo sapiens (Human) |
| Abbr | CHO-HuSCN5A |
| Alias | SCN5A, CMD1E, CDCD2, CMPD2, HB1, HB2, HBBD, HH1, ICCD, IVF, LQT3, Nav1.5, PFHB1, SSS1, VF1, CMD1E |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Applications |
1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research |
| Size | >1x106 frozen cells/vial |
| Stability | Validated for at least 10 passages |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Storage | Liquid nitrogen |
| Shipping | Dry ice |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Morphology | Epithelial-like |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
| Gene Name | SCN5A sodium channel, voltage-gated, type V, alpha subunit [ Homo sapiens ] |
| Gene Symbol | SCN5A |
| Synonyms | HB1; HB2; HH1; IVF; VF1; HBBD; ICCD; LQT3; SSS1; CDCD2; CMD1E; CMPD2; PFHB1; Nav1.5 |
| Gene Description | sodium channel, voltage-gated, type V, alpha subunit |
| GeneID | 6331 |
| Uni ProtID | Q14524 |
| mRNA Refseq | NM_000335.4 |
| Protein Refseq | NP_000326.2 |
| Chromosome Location | 3p21 |
| Function | ankyrin binding; calmodulin binding; ion channel binding; protein binding; scaffold protein binding; voltage-gated sodium channel activity; voltage-gated sodium channel activity involved in regulation of cardiac muscle cell action potential; voltage-gated sodium channel activity involved in regulation of cardiac muscle cell action potential; |
| Pathway | Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Interaction between L1 and Ankyrins, organism-specific biosystem; L1CAM interactions, organism-specific biosystem; SIDS Susceptibility Pathways, organism-specific biosystem; |
| MIM | 600163 |
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
The Nav1.5 CHO-K1 line from Creative Biogene provided incredibly clean electrophysiology data with minimal background leak. The cells recover quickly post-thaw and are a breeze to culture. It perfectly meets our stringent requirements for high-throughput screening.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.