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Cat.No. : CSC-RO0260 Host Cell: HEK293T
| Cat. No. | CSC-RO0260 |
| Description | Human GPC3 Stable Cell Line - HEK293 is engineered to stably overexpress human glypican 3 (GPC3). |
| Gene | GPC3 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human GPC3 Stable Cell Line - HEK293 is engineered to express the Glypican-3 (GPC3) protein, which is overexpressed in various cancers, including hepatocellular carcinoma. This cell line serves as a model to study the role of GPC3 in cancer progression, metastasis, and response to therapeutic interventions, contributing to the development of targeted therapies.
A: The Human GPC3 Stable Cell Line - HEK293 is specifically designed to express GPC3, a protein that is not typically expressed in standard HEK293 cells. This makes it a unique tool for high-throughput screening of compounds that modulate GPC3 activity, which could lead to the discovery of new cancer therapeutics.
A: The use of the Human GPC3 Stable Cell Line - HEK293 must adhere to ethical guidelines that protect human dignity and welfare. This includes obtaining informed consent for the use of human-derived materials, ensuring that the cell line is used solely for research purposes, and following guidelines set by institutional review boards (IRBs) and relevant regulatory bodies.
A: Researchers can utilize the Human GPC3 Stable Cell Line - HEK293 to investigate the molecular pathways involved in GPC3 expression and function. This includes studying the effects of various signaling molecules, transcription factors, and epigenetic modifications on GPC3 regulation.
A: By providing a controlled environment to study GPC3 expression and function, the Human GPC3 Stable Cell Line - HEK293 can help researchers investigate the role of GPC3 in cell signaling pathways, its involvement in cancer development, and its potential as a therapeutic target.
A: The Human GPC3 Stable Cell Line - HEK293 should be stored in a frozen state at temperatures below -80°C to preserve its stability. For revival, the cells should be thawed rapidly in a water bath at 37°C and then transferred to a culture medium appropriate for HEK293 cells, ensuring that the cells are gradually warmed to room temperature before being placed in a cell culture incubator.
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