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Cat.No. : CSC-RO0294 Host Cell: HEK293T
| Cat. No. | CSC-RO0294 |
| Description | This cell line is derived from HEK293T and is engineered to stably overexpress Human GPC3. |
| Gene | GPC3 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: For drug screening, the culture conditions of the Human GPC3 Stable Cell Line - HEK293T should be optimized to ensure stable expression of GPC3. Additionally, screening methods suitable for evaluating tumor cell proliferation, apoptosis, and migration should be chosen to identify drugs that effectively modulate GPC3 activity.
A: The Human GPC3 Stable Cell Line - HEK293T has promising prospects in simulating the liver cancer microenvironment. By culturing these cells under specific conditions, the liver cancer microenvironment can be simulated, studying the role of GPC3 in the interaction between tumor cells and the microenvironment.
A: When conducting gene editing experiments with the Human GPC3 Stable Cell Line - HEK293T, it is important to maintain the expression and function of the GPC3 protein. The choice and design of gene editing tools, such as CRISPR/Cas9, should ensure specificity to the target gene and accurate validation of the editing effects.
A: The Human GPC3 Stable Cell Line - HEK293T has potential applications in studying immunotherapy targets. Due to the high expression of GPC3 in various tumors, this cell line can be used to evaluate immunotherapeutic strategies targeting GPC3, such as antibody-drug conjugates (ADCs) and CAR-T cell therapy.
A: By using the Human GPC3 Stable Cell Line - HEK293T, various biochemical and molecular biology experiments can be conducted to study the role of GPC3 in liver cell proliferation and differentiation. For example, gene silencing or overexpression techniques can be used to investigate the impact of changes in GPC3 expression on the biological characteristics of liver cells.
A: The Human GPC3 Stable Cell Line - HEK293T, due to its stable expression of human glypican-3 (GPC3), has significant potential in liver cancer research. GPC3 is an important marker on the surface of liver cancer cells, and this cell line can be used to study the role of GPC3 in the initiation, progression, and metastasis of liver cancer, as well as to evaluate therapeutic strategies targeting GPC3.
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