Human GPC3 Stable Cell Line - HEK293T

Name: Human GPC3 Stable Cell Line - HEK293T
SKU: CSC-RO0294
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0294

Host Cell : HEK293T Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0294
Description	This cell line is derived from HEK293T and is engineered to stably overexpress Human GPC3.
Target Gene	GPC3
Gene Species	Homo sapiens (Human)
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	GPC3 glypican 3 [ Homo sapiens ]
Gene Symbol	GPC3
Synonyms	GPC3; glypican 3; SDYS; glypican-3; DGSX; glypican proteoglycan 3; OCI 5; SGB; SGBS; SGBS1; secreted glypican-3; intestinal protein OCI-5; heparan sulphate proteoglycan; MXR7; OCI-5; GTR2-2;
Gene ID	2719
Uni Prot ID	P51654
m RNA Refseq	BC035972
Chromosome Location	Xq26
Function	heparan sulfate proteoglycan binding; peptidyl-dipeptidase inhibitor activity; protein binding;
Pathway	Glypican 3 network, organism-specific biosystem; Glypican pathway, organism-specific biosystem;
MIM	300037

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Q & A

Customer Reviews

Customer Q&As

How should experimental conditions be optimized for drug screening using the Human GPC3 Stable Cell Line - HEK293T?

A: For drug screening, the culture conditions of the Human GPC3 Stable Cell Line - HEK293T should be optimized to ensure stable expression of GPC3. Additionally, screening methods suitable for evaluating tumor cell proliferation, apoptosis, and migration should be chosen to identify drugs that effectively modulate GPC3 activity.

What are the prospects of the Human GPC3 Stable Cell Line - HEK293T in simulating the liver cancer microenvironment?

A: The Human GPC3 Stable Cell Line - HEK293T has promising prospects in simulating the liver cancer microenvironment. By culturing these cells under specific conditions, the liver cancer microenvironment can be simulated, studying the role of GPC3 in the interaction between tumor cells and the microenvironment.

What key factors should be considered when conducting gene editing experiments with the Human GPC3 Stable Cell Line - HEK293T?

A: When conducting gene editing experiments with the Human GPC3 Stable Cell Line - HEK293T, it is important to maintain the expression and function of the GPC3 protein. The choice and design of gene editing tools, such as CRISPR/Cas9, should ensure specificity to the target gene and accurate validation of the editing effects.

What is the potential application of the Human GPC3 Stable Cell Line - HEK293T in studying immunotherapy targets?

A: The Human GPC3 Stable Cell Line - HEK293T has potential applications in studying immunotherapy targets. Due to the high expression of GPC3 in various tumors, this cell line can be used to evaluate immunotherapeutic strategies targeting GPC3, such as antibody-drug conjugates (ADCs) and CAR-T cell therapy.

How can the Human GPC3 Stable Cell Line - HEK293T be used to study the role of GPC3 in liver cell proliferation and differentiation?

A: By using the Human GPC3 Stable Cell Line - HEK293T, various biochemical and molecular biology experiments can be conducted to study the role of GPC3 in liver cell proliferation and differentiation. For example, gene silencing or overexpression techniques can be used to investigate the impact of changes in GPC3 expression on the biological characteristics of liver cells.

What is the potential application of the Human GPC3 Stable Cell Line - HEK293T in liver cancer research?

A: The Human GPC3 Stable Cell Line - HEK293T, due to its stable expression of human glypican-3 (GPC3), has significant potential in liver cancer research. GPC3 is an important marker on the surface of liver cancer cells, and this cell line can be used to study the role of GPC3 in the initiation, progression, and metastasis of liver cancer, as well as to evaluate therapeutic strategies targeting GPC3.

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