Mouse 4-1BB Stable Cell Line-CHO-K1

Name: Mouse 4-1BB Stable Cell Line-CHO-K1
SKU: CSC-RO0065
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0065

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0065
Description	This cell line is a stably transfected cell line which expresses mouse tumor necrosis factor receptor superfamily, member 9 (Tnfrsf9) without any tag.
Background	Homozygous mutation of this gene results in enhanced T cell proliferation, decreased B cell IgG production, decreased cytotoxic T cell activity, and increased numbers of erythrocytes, granulocyte macrophages, and multipotential progenitor cells in the bone marrow, blood, and spleen.
Target	Mouse
Target Gene	Tnfrsf9
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Source	CHO-K1
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	Tnfrsf9 tumor necrosis factor receptor superfamily, member 9 [ Mus musculus (house mouse) ]
Gene Symbol	Tnfrsf9
Synonyms	ILA; Ly63; 4-1BB; Cd137; CDw137; AA408498; AI325004; A930040I11Rik
GeneID	21942
Uni ProtID	P20334
Chromosome Location	4 E2; 4 81.52 cM

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

What is 4-1BB?

A: 4-1BB is a member of the tumor necrosis factor (TNF) receptor family.

Does 4-1BB have other names?

A: Other names for 4-1BB include Tumor Necrosis Factor Receptor Superfamily Member 9 (TNFRSF9), CD137, and Inducible Lymphocyte Activation (ILA).

How is 4-1BB expressed?

A: 4-1BB is expressed by activated T cells, but the expression level on CD8 is greater than on CD4 T cells.

What types of cells can express 4-1BB?

A: 4-1BB expression is found on dendritic cells at inflammatory sites, follicular dendritic cells, natural killer cells, granulocytes, and vascular wall cells.

How to verify the protein expression on the surface of Mouse 4-1BB Stable Cell Line-CHO cells?

A: Cell surface protein expression can be verified by FACS or WB.

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