Cat.No. : CSC-RO0002 Host Cell: CHO-K1
| Cat. No. | CSC-RO0002 |
| Description | This cell line is engineered to stably overexpress the human CD276 in CHO-K1 cells. |
| Gene | CD276 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Gene Name | CD276 CD276 molecule [ Homo sapiens ] |
| Gene Symbol | CD276 |
| Synonyms | B7H3; B7-H3; B7RP-2; 4Ig-B7-H3 |
| Gene Description | CD276 molecule |
| Gene ID | 80381 |
| UniProt ID | Q5ZPR3 |
| mRNA Refseq | NM_025240.2 |
| Protein Refseq | NP_079516.1 |
| Chromosome Location | 15q23-q24 |
| Function | protein binding; receptor binding; |
| Pathway | Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; |
| MIM | 605715 |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human CD276 Stable Cell Line-CHO was developed by transfecting CHO cells with a vector containing the human CD276 gene followed by selection with an appropriate antibiotic. Stable integration was confirmed using genomic PCR and Southern blot analysis.
A: Yes, the Human CD276 Stable Cell Line-CHO can be used for high-throughput screening assays. Its advantages include consistent expression of CD276, allowing for reproducible results across multiple experiments and plates.
A: The Human CD276 Stable Cell Line-CHO has an enhanced expression level of CD276 protein compared to wild-type CHO cells, which can be quantified using techniques like Western blotting or flow cytometry.
A: The Human CD276 Stable Cell Line-CHO requires standard mammalian cell culture conditions with the addition of a specific selection antibiotic to maintain stable expression.
A: Functionality can be validated using binding assays with CD276 ligands or by studying the downstream signaling pathways activated by CD276 in the Human CD276 Stable Cell Line-CHO.
A: The morphology of the Human CD276 Stable Cell Line-CHO is generally similar to untransfected CHO cells, but researchers should monitor the cells for any subtle changes that might arise from CD276 overexpression.
A: The Human CD276 Stable Cell Line-CHO can be used in co-culture experiments with immune cells to study interactions and modulation. Specific data would need to be referenced from scientific publications or the product's manufacturer documentation.
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