Human CD276 Stable Cell Line-CHO-K1

Name: Human CD276 Stable Cell Line-CHO-K1
SKU: CSC-RO0002
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0002

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0002
Description	This cell line is engineered to stably overexpress the human CD276 in CHO-K1 cells.
Target Gene	CD276
Gene Species	Homo sapiens (Human)
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Source	CHO-K1
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CD276 CD276 molecule [ Homo sapiens ]
Gene Symbol	CD276
Synonyms	B7H3; B7-H3; B7RP-2; 4Ig-B7-H3
Gene Description	CD276 molecule
GeneID	80381
Uni ProtID	Q5ZPR3
mRNA Refseq	NM_025240.2
Protein Refseq	NP_079516.1
Chromosome Location	15q23-q24
Function	protein binding; receptor binding;
Pathway	Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem;
MIM	605715

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Background

Q & A

Customer Reviews

The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Customer Q&As

How was the Human CD276 Stable Cell Line-CHO developed and what methods were employed to ensure stable integration of the CD276 gene?

A: The Human CD276 Stable Cell Line-CHO was developed by transfecting CHO cells with a vector containing the human CD276 gene followed by selection with an appropriate antibiotic. Stable integration was confirmed using genomic PCR and Southern blot analysis.

Can the Human CD276 Stable Cell Line-CHO be used for high-throughput screening assays, and if so, what would be its advantages?

A: Yes, the Human CD276 Stable Cell Line-CHO can be used for high-throughput screening assays. Its advantages include consistent expression of CD276, allowing for reproducible results across multiple experiments and plates.

What is the expression level of CD276 protein in the Human CD276 Stable Cell Line-CHO compared to wild-type CHO cells?

A: The Human CD276 Stable Cell Line-CHO has an enhanced expression level of CD276 protein compared to wild-type CHO cells, which can be quantified using techniques like Western blotting or flow cytometry.

In terms of culturing, are there any special requirements for maintaining the Human CD276 Stable Cell Line-CHO?

A: The Human CD276 Stable Cell Line-CHO requires standard mammalian cell culture conditions with the addition of a specific selection antibiotic to maintain stable expression.

How can researchers validate the functionality of CD276 expressed in the Human CD276 Stable Cell Line-CHO?

A: Functionality can be validated using binding assays with CD276 ligands or by studying the downstream signaling pathways activated by CD276 in the Human CD276 Stable Cell Line-CHO.

Does the Human CD276 Stable Cell Line-CHO show any unique morphological characteristics compared to untransfected CHO cells?

A: The morphology of the Human CD276 Stable Cell Line-CHO is generally similar to untransfected CHO cells, but researchers should monitor the cells for any subtle changes that might arise from CD276 overexpression.

Is there any available data on how the Human CD276 Stable Cell Line-CHO interacts with immune cells or its role in immune modulation?

A: The Human CD276 Stable Cell Line-CHO can be used in co-culture experiments with immune cells to study interactions and modulation. Specific data would need to be referenced from scientific publications or the product's manufacturer documentation.

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