Cat.No. : CSC-RO0009 Host Cell: CHO-K1
| Cat. No. | CSC-RO0009 |
| Description | This cell line is engineered to stably overexpress the human LAG3 in CHO-K1 cells. |
| Background | Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. |
| Gene | LAG3 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Gene Name | LAG3 lymphocyte-activation gene 3 [ Homo sapiens ] |
| Gene Symbol | LAG3 |
| Synonyms | CD223 |
| Gene Description | lymphocyte-activation gene 3 |
| Gene ID | 3902 |
| UniProt ID | P18627 |
| mRNA Refseq | NM_002286.5 |
| Protein Refseq | NP_002277.4 |
| Chromosome Location | 12p13.32 |
| Function | MHC class II protein binding; antigen binding; transmembrane signaling receptor activity; |
| MIM | 153337 |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human LAG3 Stable Cell Line-CHO, by stably expressing LAG-3 protein, provides a model for studying immune suppression mechanisms. LAG-3, as an immune checkpoint molecule, plays a negative regulatory role in T cell activation. This cell line can help scientists understand how LAG-3 affects the activation state of T cells and its role in immune escape and tumor microenvironments, which is significant for developing new immunotherapeutic strategies.
A: By using the Human LAG3 Stable Cell Line-CHO, researchers can assess the efficacy of antibodies or small molecule drugs targeting LAG-3. This cell line can simulate the expression of LAG-3 in the human body, helping researchers understand how these drugs bind to LAG-3 and evaluate their impact on cell function, which is crucial for drug development and clinical application.
A: The Human LAG-3 Stable Cell Line-CHO can be used to study the abnormal behavior of T cells in autoimmune diseases. By expressing LAG-3 in this cell line, researchers can simulate how self-antigens are recognized by T cells and how this recognition leads to autoimmune reactions. This is important for understanding the pathogenesis of autoimmune diseases and developing new treatment strategies.
A: The Human LAG-3 Stable Cell Line-CHO provides a model system for studying the molecular mechanisms of T cell receptor (TCR) signaling. By expressing LAG-3 in this cell line, researchers can investigate the activation of downstream signaling pathways after TCR activation, which is significant for understanding T cell function and regulation.
A: The Human LAG3 Stable Cell Line-CHO can be used to study the mechanisms of immune tolerance mediated by T cells. By expressing LAG-3 in this cell line, researchers can simulate how T cells recognize self-tissues and maintain immune tolerance. This is important for preventing the onset of autoimmune diseases.
A: The role of the Human LAG3 Stable Cell Line-CHO in studying tumor immune escape lies in simulating the immune suppressive mechanisms in the tumor microenvironment. The expression of LAG-3 in the tumor microenvironment can lead to T cell dysfunction, helping tumor cells evade immune surveillance. This cell line can be used to study how tumor cells upregulate LAG-3 to suppress T cell activity, which is significant for developing new tumor immunotherapies.
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