Human LAG3 Stable Cell Line-CHO-K1

Name: Human LAG3 Stable Cell Line-CHO-K1
SKU: CSC-RO0009
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0009

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0009
Description	This cell line is engineered to stably overexpress the human LAG3 in CHO-K1 cells.
Background	Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4.
Target Gene	LAG3
Gene Species	Homo sapiens (Human)
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Source	CHO-K1
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	LAG3 lymphocyte-activation gene 3 [ Homo sapiens ]
Gene Symbol	LAG3
Synonyms	CD223
Gene Description	lymphocyte-activation gene 3
GeneID	3902
Uni ProtID	P18627
mRNA Refseq	NM_002286.5
Protein Refseq	NP_002277.4
Chromosome Location	12p13.32
Function	MHC class II protein binding; antigen binding; transmembrane signaling receptor activity;
MIM	153337

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

What is the role of the Human LAG3 Stable Cell Line-CHO in studying immune suppression mechanisms?

A: The Human LAG3 Stable Cell Line-CHO, by stably expressing LAG-3 protein, provides a model for studying immune suppression mechanisms. LAG-3, as an immune checkpoint molecule, plays a negative regulatory role in T cell activation. This cell line can help scientists understand how LAG-3 affects the activation state of T cells and its role in immune escape and tumor microenvironments, which is significant for developing new immunotherapeutic strategies.

How does the Human LAG3 Stable Cell Line-CHO contribute to evaluating the efficacy of LAG-3 targeted therapeutics?

A: By using the Human LAG3 Stable Cell Line-CHO, researchers can assess the efficacy of antibodies or small molecule drugs targeting LAG-3. This cell line can simulate the expression of LAG-3 in the human body, helping researchers understand how these drugs bind to LAG-3 and evaluate their impact on cell function, which is crucial for drug development and clinical application.

How is the Human LAG3 Stable Cell Line-CHO applied in studying autoimmune diseases?

A: The Human LAG-3 Stable Cell Line-CHO can be used to study the abnormal behavior of T cells in autoimmune diseases. By expressing LAG-3 in this cell line, researchers can simulate how self-antigens are recognized by T cells and how this recognition leads to autoimmune reactions. This is important for understanding the pathogenesis of autoimmune diseases and developing new treatment strategies.

What is the role of the Human LAG3 Stable Cell Line-CHO in studying T cell receptor signaling?

A: The Human LAG-3 Stable Cell Line-CHO provides a model system for studying the molecular mechanisms of T cell receptor (TCR) signaling. By expressing LAG-3 in this cell line, researchers can investigate the activation of downstream signaling pathways after TCR activation, which is significant for understanding T cell function and regulation.

What is the role of the Human LAG3 Stable Cell Line-CHO in studying immune tolerance?

A: The Human LAG3 Stable Cell Line-CHO can be used to study the mechanisms of immune tolerance mediated by T cells. By expressing LAG-3 in this cell line, researchers can simulate how T cells recognize self-tissues and maintain immune tolerance. This is important for preventing the onset of autoimmune diseases.

What is the role of the Human LAG3 Stable Cell Line-CHO in studying tumor immune escape?

A: The role of the Human LAG3 Stable Cell Line-CHO in studying tumor immune escape lies in simulating the immune suppressive mechanisms in the tumor microenvironment. The expression of LAG-3 in the tumor microenvironment can lead to T cell dysfunction, helping tumor cells evade immune surveillance. This cell line can be used to study how tumor cells upregulate LAG-3 to suppress T cell activity, which is significant for developing new tumor immunotherapies.

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