Human CD274 Stable Cell Line-HEK293T

Name: Human CD274 Stable Cell Line-HEK293T
SKU: CSC-RO0012

Cat.No. : CSC-RO0012 Host Cell: HEK293T

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Cell Line Information

Cell Culture Information

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Gene Informationn

Cat. No.	CSC-RO0012
Description	This cell line is engineered to stably overexpress the human CD274 in HEK293T cells.
Gene	CD274
Gene Species	Homo sapiens (Human)
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Stability	Validated for at least 10 passages
Application	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Shipping	Dry ice
Storage	Liquid nitrogen

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm² flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Gene Name	CD274 CD274 molecule [ Homo sapiens ]
Gene Symbol	CD274
Synonyms	CD274; CD274 molecule; CD274 antigen , PDCD1LG1, programmed cell death 1 ligand 1; programmed cell death 1 ligand 1; B7 homolog 1; B7 H; B7 H1; B7H1; PD L1; PDL1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1; B7-H; PD-L1; PDCD1L1; PDCD1LG1; MGC142294; MGC142296;
Gene ID	29126
UniProt ID	Q9NZQ7
mRNA Refseq	BC113734
Chromosome Location	9p24.1
Function	protein binding; protein tyrosine phosphatase activity; receptor activity;
Pathway	Adaptive Immune System, organism-specific biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Costimulation by the CD28 family, organism-specific biosystem; Immune System, organism-specific biosystem; PD-1 signaling, organism-specific biosystem;
MIM	605402

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Background

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This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants.

Customer Q&As

What was the primary objective behind developing the Human CD274 Stable Cell Line-HEK293, and how does it serve in advancing the understanding of CD274 function?

A: The primary objective behind developing the Human CD274 Stable Cell Line-HEK293 was to provide a consistent and reliable model system for studying the expression, regulation, and function of CD274. This cell line aids researchers in understanding the role of CD274 in immune checkpoint regulation and other cellular processes.

How do the expression levels of CD274 in Human CD274 Stable Cell Line-HEK293 compare to native HEK293 cells without the modification?

A: The Human CD274 Stable Cell Line-HEK293 exhibits enhanced and consistent expression of CD274 compared to native HEK293 cells, which can be quantified using methods like Western blotting or flow cytometry.

Can the Human CD274 Stable Cell Line-HEK293 be employed for drug screening, especially for potential immune checkpoint inhibitors?

A: Yes, the Human CD274 Stable Cell Line-HEK293 can be effectively used for drug screening. Its consistent expression of CD274 makes it an ideal candidate for evaluating potential immune checkpoint inhibitors or other therapeutic compounds targeting CD274.

What are the culturing conditions recommended for the Human CD274 Stable Cell Line-HEK293 to maintain its stable phenotype and ensure optimal CD274 expression?

A: The Human CD274 Stable Cell Line-HEK293 should be cultured under standard mammalian cell culture conditions, with the addition of a specific selection antibiotic to ensure stable CD274 expression and maintain the modified phenotype.

Are there any reported morphological or growth rate differences in the Human CD274 Stable Cell Line-HEK293 when compared to the standard HEK293 line?

A: While the Human CD274 Stable Cell Line-HEK293 is designed to primarily alter CD274 expression, researchers should routinely monitor the cells. Any significant morphological or growth rate changes would be product-specific and should be detailed in the product's accompanying documentation.

How do researchers typically validate the functional activity of CD274 in the Human CD274 Stable Cell Line-HEK293?

A: To validate the functionality of CD274 in the Human CD274 Stable Cell Line-HEK293, researchers can utilize ligand-binding assays, co-culture assays with T cells to assess immune cell interactions, or investigate downstream signaling pathways triggered by CD274.

Have there been any studies or publications utilizing the Human CD274 Stable Cell Line-HEK293 to elucidate the molecular mechanisms of CD274-mediated immune modulation?

A: Specific studies or publications would be dependent on the research community's usage. Researchers interested in such data should consult scientific literature databases or reach out to the product's manufacturer for references.

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