Cat.No. : CSC-RO0615 Host Cell: CT26
| Cat. No. | CSC-RO0615 |
| Description | This cell line is derived from CT26 and is engineered to stably overexpress Human GPC3. |
| Gene | GPC3 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | CT26 |
| Host Cell Species | Mus musculus (Mouse) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Glypican 3 (GPC3) is a member of the heparan sulfate proteoglycan (HSPG) family of glypicans. The GPC3 gene may play a role in controlling cell migration, negatively regulating cell growth, and inducing apoptosis. GPC3 is downregulated in several cancers, which may lead to uncontrolled cell growth and may also contribute to the malignant phenotype of some tumors. Here, researchers observed downregulation of GPC3 in clear cell renal cell carcinoma samples and cell lines compared with normal kidney samples. GPC3 mRNA expression and protein levels increased in 786-O and ACHN cell lines after transfection with a GPC3 expression construct, and cell proliferation rates were reduced in both cell lines after GPC3 overexpression. In addition, apoptosis was not induced in renal cell carcinoma cell lines overexpressing GPC3, and the population of cells in the G1 phase of the cell cycle increased. Therefore, the GPC3 gene reduces cell proliferation rate by arresting the cell cycle at the G1 phase in renal cell carcinoma.
Cell proliferation of ACHN and 786-O cells overexpressing GPC3 was measured by MTT assay 24, 48, 72, 96, and 120 hours after transfection. Compared with ACHN cells lacking GPC3 expression, ACHN cells overexpressing GPC3 showed a significant decrease in cell proliferation after 48, 72, 96, and 120 hours (Figure 1A). 786-O cells overexpressing GPC3 also showed a decrease in proliferation after 48, 72, 96, and 120 hours (Figure 1B).
Figure 1. Effect of GPC3 on cell proliferation. A) GPC3 over-expressing ACHN cells experienced a significant reduction in proliferation rate after 48 h, 72, 96 and 120 h. B) GPC3 over-expressing 786-O cells experienced a significant reduction in proliferation rate after 48 h, 72 h, 96 h and 120 h. (Valsechi M C, et al., 2014)
Creative Biogene has successfully constructed GPC3 over-expressing stable cell lines with CT26, HEK293, ACHN, 786-O, CHO, HeLa, 1321N1, U2OS, RH7777, etc. These GPC3 stable cell lines have excellent in vitro assay sensitivity and reproducibility. Therefore, conducting experiments with our GPC3 stable cell lines saves time and resources while providing more accurate results.
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I've been impressed with the superior performance of the Human GPC3 Stable Cell Line - CT26 in our in vitro assays.
Integrating the Human GPC3 Stable Cell Line - CT26 with our existing experimental protocols was seamless. The product's compatibility and ease of use have meaningfully streamlined our workflow, allowing our team to focus more on data analysis and interpretation rather than troubleshooting cell line issues.
The expression of GPC3 in this stable cell line has shown enhanced target specificity in our drug screening applications. This has enabled us to effectively identify and characterize potential therapeutic candidates with greater precision, ultimately accelerating our drug development process.
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