Human HAVCR2 Stable Cell Line-CHO-K1

Name: Human HAVCR2 Stable Cell Line-CHO-K1
SKU: CSC-RO0013
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0013

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0013
Description	This cell line is engineered to stably overexpress the human HAVCR2 in CHO-K1 cells.
Background	The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance.
Target Gene	HAVCR2
Gene Species	Homo sapiens (Human)
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Source	CHO-K1
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	HAVCR2 hepatitis A virus cellular receptor 2 [ Homo sapiens ]
Gene Symbol	HAVCR2
Synonyms	HAVCR2; hepatitis A virus cellular receptor 2; FLJ14428; Tim 3; TIM3; TIMD3; kidney injury molecule-3; T-cell membrane protein 3; T cell immunoglobulin mucin 3; T cell immunoglobulin mucin-3; T-cell immunoglobulin and mucin domain-containing protein 3; KIM-3; Tim-3; TIMD-3; HAVcr-2;
GeneID	84868
Uni ProtID	Q8TDQ0
mRNA Refseq	BC063431
Chromosome Location	5q34
MIM	606652

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Q & A

Customer Reviews

Customer Q&As

What is the origin of the Human HAVCR2 Stable Cell Line - CHO, and how was it established?

A: The Human HAVCR2 Stable Cell Line - CHO is likely derived from CHO (Chinese Hamster Ovary) cells and has been genetically modified to stably express the human HAVCR2 gene. The establishment of this stable cell line typically involves transfecting CHO cells with a plasmid or viral vector containing the human HAVCR2 gene and a selectable marker, followed by the selection of cells expressing HAVCR2. This results in the creation of a stable cell line capable of consistently producing the HAVCR2 protein.

What are the specific applications of the Human HAVCR2 Stable Cell Line - CHO in scientific research?

A: The Human HAVCR2 Stable Cell Line - CHO can be used in various scientific research applications, particularly in the study of human HAVCR2, also known as Tim-3. Some potential applications include investigating the role of HAVCR2 in immune regulation, immune checkpoint pathways, and its interaction with ligands such as galectin-9. Researchers can use this cell line to study HAVCR2-mediated signaling, immune responses, and the development of therapeutics targeting the HAVCR2 pathway.

Are there any unique characteristics or advantages associated with the Human HAVCR2 Stable Cell Line - CHO compared to other cell lines expressing HAVCR2?

A: The Human HAVCR2 Stable Cell Line - CHO may offer specific advantages, such as stable and consistent expression of human HAVCR2. CHO cells are a widely used expression system known for their ability to produce recombinant proteins effectively. This cell line could be advantageous for researchers interested in studying human HAVCR2 in a controlled and reproducible environment. However, the choice of cell line should align with the specific research goals and experimental requirements.

What are the recommended culture conditions and media for maintaining the Human HAVCR2 Stable Cell Line - CHO?

A: To maintain the Human HAVCR2 Stable Cell Line - CHO, researchers should typically culture the cells in a suitable growth medium for CHO cells, supplemented with any necessary selection agents or antibiotics. The specific culture conditions, including temperature, CO2 concentration, and passaging protocols, should be in accordance with established protocols provided by the cell line provider or described in relevant literature.

What precautions should researchers take when working with the Human HAVCR2 Stable Cell Line - CHO to ensure reliable and reproducible results?

A: When working with the Human HAVCR2 Stable Cell Line - CHO, researchers should consider the following precautions: a. Regularly monitor and maintain the stability of human HAVCR2 expression to ensure consistent results.<br/>b. Confirm the presence of the selection agent or antibiotic to prevent the loss of HAVCR2-expressing cells.

Are there any known limitations or challenges associated with using the Human HAVCR2 Stable Cell Line - CHO in research, such as potential differences in post-translational modifications compared to native cells?

A: Researchers should be aware of potential limitations when using the Human HAVCR2 Stable Cell Line - CHO. One important consideration is the possibility of differences in post-translational modifications or glycosylation patterns compared to native human cells. These modifications can impact the function and binding affinity of HAVCR2. Validation experiments and comparisons with native human cells may be necessary to ensure that findings from this cell line accurately represent the biology of human HAVCR2.

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Customer Reviews

Exceptional Consistency in Binding Assays

The HAVCR2 (TIM-3) stable line has been central to our latest inhibitor screening project. The signal-to-noise ratio in our flow-based assays is fantastic. It arrived promptly, well-packaged on dry ice, and thrived immediately upon thawing.

Germany

2025-11-08

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Human HAVCR2 Stable Cell Line-CHO-K1

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Q & A

Customer Reviews

What is the origin of the Human HAVCR2 Stable Cell Line - CHO, and how was it established?

What are the specific applications of the Human HAVCR2 Stable Cell Line - CHO in scientific research?

Are there any unique characteristics or advantages associated with the Human HAVCR2 Stable Cell Line - CHO compared to other cell lines expressing HAVCR2?

What are the recommended culture conditions and media for maintaining the Human HAVCR2 Stable Cell Line - CHO?

What precautions should researchers take when working with the Human HAVCR2 Stable Cell Line - CHO to ensure reliable and reproducible results?

Are there any known limitations or challenges associated with using the Human HAVCR2 Stable Cell Line - CHO in research, such as potential differences in post-translational modifications compared to native cells?

Exceptional Consistency in Binding Assays

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