Human CXCR4-SNAP Stable Cell Line-HEK293

Name: Human CXCR4-SNAP Stable Cell Line-HEK293
SKU: CSC-RG0043
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0043

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0043
Background	This gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Target Gene	CXCR4
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuCXCR4-SNAP
Alias	CXCR4, CD184, HM89, HSY3RR, LESTR, NPY3R, NPYR, NPYY3R, CXC-R4, CXCR-4, FB22, LAP3, LCR1
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CXCR4 chemokine (C-X-C motif) receptor 4 [ Homo sapiens ]
Gene Symbol	CXCR4
Synonyms	FB22; HM89; LAP3; LCR1; NPYR; WHIM; CD184; LESTR; NPY3R; NPYRL; HSY3RR; NPYY3R; D2S201E
Gene Description	chemokine (C-X-C motif) receptor 4
Gene ID	7852
Uni Prot ID	P61073
m RNA Refseq	NM_001008540.1
Protein Refseq	NP_001008540.1
Chromosome Location	2q21
Function	C-X-C chemokine receptor activity; G-protein coupled receptor activity; actin binding; coreceptor activity; myosin light chain binding; protein binding; ubiquitin binding; ubiquitin protein ligase binding;
Pathway	Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; Binding and entry of HIV virion, organism-specific biosystem; CXCR4-mediated signaling events, organism-specific biosystem; Chemokine receptors bind chemokines, organism-specific biosystem; Chemokine signaling pathway, organism-specific biosystem; Chemokine signaling pathway, conserved biosystem;
MIM	162643

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Customer Q&As

What is the purpose of stably expressing Human CXCR4-SNAP in the HEK293 cell line?

A: The purpose of stably expressing Human CXCR4-SNAP in the HEK293 cell line is to study the function of the CXCR4 receptor, particularly through protein labeling and capture facilitated by the SNAP tag (single-chain affinity purification tag). This cell line allows researchers to capture proteins interacting with the CXCR4 receptor using chemical methods, thereby revealing its signaling pathways and potential regulatory mechanisms.

What is the application of the Human CXCR4-SNAP Stable Cell Line-HEK293 in drug screening?

A: This cell line can be used for high-throughput screening (HTS) and drug discovery by monitoring changes in the activity of the CXCR4 receptor to assess the efficacy of potential drug molecules. For example, compounds that can activate or inhibit the activity of the CXCR4 receptor can be screened, which is significant for developing drugs to treat HIV infection and certain cancers.

How can the stable expression of the CXCR4 receptor in the Human CXCR4-SNAP Stable Cell Line-HEK293 be ensured?

A: To ensure stable expression of the CXCR4 receptor, researchers typically use a selective marker gene (such as an antibiotic resistance gene) to select cells that have successfully integrated the CXCR4-SNAP expression vector. Additionally, regular phenotypic analysis and functional assays are used to monitor the expression and function of the CXCR4 receptor to ensure its stability in the cell line.

What is the role of the Human CXCR4-SNAP Stable Cell Line-HEK293 in studying the endocytosis of the CXCR4 receptor?

A: This cell line can be used to study the endocytosis of the CXCR4 receptor because the SNAP tag allows the capture of endocytic vesicles through chemical methods. This helps researchers understand how the CXCR4 receptor cycles within the cell and the proteins involved in the endocytic process.

How can the Human CXCR4-SNAP Stable Cell Line-HEK293 be utilized to study the signal transduction of the CXCR4 receptor?

A: Functional experiments, such as monitoring changes in intracellular calcium ion concentrations, can be conducted in this cell line to study signal transduction following the activation of the CXCR4 receptor. Additionally, observing the distribution and dynamic changes of the SNAP-tagged CXCR4 receptor within the cell using confocal microscopy can further elucidate its signaling mechanisms.

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