Cat.No. : CSC-RG0043 Host Cell: HEK293
| Cat. No. | CSC-RG0043 |
| Background | This gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. |
| Gene | CXCR4 |
| Gene Species | Homo sapiens (Human) |
| Alias | CXCR4, CD184, HM89, HSY3RR, LESTR, NPY3R, NPYR, NPYY3R, CXC-R4, CXCR-4, FB22, LAP3, LCR1 |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Morphology | Epithelial |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The purpose of stably expressing Human CXCR4-SNAP in the HEK293 cell line is to study the function of the CXCR4 receptor, particularly through protein labeling and capture facilitated by the SNAP tag (single-chain affinity purification tag). This cell line allows researchers to capture proteins interacting with the CXCR4 receptor using chemical methods, thereby revealing its signaling pathways and potential regulatory mechanisms.
A: This cell line can be used for high-throughput screening (HTS) and drug discovery by monitoring changes in the activity of the CXCR4 receptor to assess the efficacy of potential drug molecules. For example, compounds that can activate or inhibit the activity of the CXCR4 receptor can be screened, which is significant for developing drugs to treat HIV infection and certain cancers.
A: To ensure stable expression of the CXCR4 receptor, researchers typically use a selective marker gene (such as an antibiotic resistance gene) to select cells that have successfully integrated the CXCR4-SNAP expression vector. Additionally, regular phenotypic analysis and functional assays are used to monitor the expression and function of the CXCR4 receptor to ensure its stability in the cell line.
A: This cell line can be used to study the endocytosis of the CXCR4 receptor because the SNAP tag allows the capture of endocytic vesicles through chemical methods. This helps researchers understand how the CXCR4 receptor cycles within the cell and the proteins involved in the endocytic process.
A: Functional experiments, such as monitoring changes in intracellular calcium ion concentrations, can be conducted in this cell line to study signal transduction following the activation of the CXCR4 receptor. Additionally, observing the distribution and dynamic changes of the SNAP-tagged CXCR4 receptor within the cell using confocal microscopy can further elucidate its signaling mechanisms.
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