Human CHRM3 Stable Cell Line-U2OS

Name: Human CHRM3 Stable Cell Line-U2OS
SKU: CSC-RG0014
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0014

Host Cell : U2OS Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0014
Background	Muscarinic acetylcholine receptors are G protein-coupled receptors. M1, M3, M5 receptors couple to G proteins of the Gq/11 family, which activate phospholipase C. M2 and M4 receptors couple to Gi/o-type G proteins that inhibit adenylyl cyclase activity.Muscarinic receptors control many effects of acetylcholine in the central and peripheral nervous system. M3 receptor is found in smooth muscles, the endocrine glands, the exocrine glands, as well as the lungs. It is also found in the CNS, where it induces emesis. In general, it causes smooth muscle contraction and increased glandular secretions. M3 receptor is thought to be implicated in Alzheimer′s disease.
Target Gene	CHRM3
Gene Species	Homo sapiens (Human)
Abbr	U2OS-HuCHRM3
Alias	CHRM3, EGBRS, HM3
Host Cell	U2OS
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CHRM3 cholinergic receptor, muscarinic 3 [ Homo sapiens ]
Gene Symbol	CHRM3
Synonyms	HM3; EGBRS
Gene Description	cholinergic receptor, muscarinic 3
GeneID	1131
Uni ProtID	P20309
mRNA Refseq	NM_000740.2
Protein Refseq	NP_000731.1
Chromosome Location	1q43
Function	G-protein coupled acetylcholine receptor activity; acetylcholine binding; drug binding; phosphatidylinositol phospholipase C activity; receptor activity;
Pathway	Amine ligand-binding receptors, organism-specific biosystem; Calcium Regulation in the Cardiac Cell, organism-specific biosystem; Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Cholinergic synapse, organism-specific biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem;
MIM	118494

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Customer Q&As

What is the effect of CHRM3 receptor activation on cell proliferation in the Human CHRM3 Stable Cell Line-U2OS?

A: Activation of the CHRM3 receptor may affect the proliferation of U2OS cells through various signaling pathways. The specific effect depends on the level of activation and the cellular environment, which could include either an increase or decrease in cell proliferation.

How does the antagonist of the CHRM3 receptor affect cell migration in the Human CHRM3 Stable Cell Line-U2OS?

A: CHRM3 receptor antagonists may reduce cell migration. Since CHRM3 is associated with signaling pathways related to cell migration, antagonists might reduce migration by affecting these pathways.

How can the expression level of the CHRM3 receptor in the Human CHRM3 Stable Cell Line-U2OS be quantified?

A: The expression level of the CHRM3 receptor can be quantified by real-time quantitative PCR (qPCR) or Western Blot to measure mRNA or protein levels, respectively.

What types of drug screening is the Human CHRM3 Stable Cell Line-U2OS most suitable for?

A: This cell line is suitable for drug screening related to the CHRM3 receptor, including agonists, antagonists, and modulators.

How does the Human CHRM3 Stable Cell Line-U2OS respond to hypoxic conditions?

A: Hypoxic conditions might affect the function and expression of the CHRM3 receptor, thereby impacting cell survival, proliferation, and migration. The specific effects need to be determined experimentally.

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