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Cat.No. : CSC-RG0014 Host Cell: U2OS
| Cat. No. | CSC-RG0014 |
| Background | Muscarinic acetylcholine receptors are G protein-coupled receptors. M1, M3, M5 receptors couple to G proteins of the Gq/11 family, which activate phospholipase C. M2 and M4 receptors couple to Gi/o-type G proteins that inhibit adenylyl cyclase activity.Muscarinic receptors control many effects of acetylcholine in the central and peripheral nervous system. M3 receptor is found in smooth muscles, the endocrine glands, the exocrine glands, as well as the lungs. It is also found in the CNS, where it induces emesis. In general, it causes smooth muscle contraction and increased glandular secretions. M3 receptor is thought to be implicated in Alzheimer′s disease. |
| Gene | CHRM3 |
| Gene Species | Homo sapiens (Human) |
| Alias | CHRM3, EGBRS, HM3 |
| Host Cell | U2OS |
| Host Cell Species | Homo sapiens (Human) |
| Morphology | Epithelial |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: Activation of the CHRM3 receptor may affect the proliferation of U2OS cells through various signaling pathways. The specific effect depends on the level of activation and the cellular environment, which could include either an increase or decrease in cell proliferation.
A: CHRM3 receptor antagonists may reduce cell migration. Since CHRM3 is associated with signaling pathways related to cell migration, antagonists might reduce migration by affecting these pathways.
A: The expression level of the CHRM3 receptor can be quantified by real-time quantitative PCR (qPCR) or Western Blot to measure mRNA or protein levels, respectively.
A: This cell line is suitable for drug screening related to the CHRM3 receptor, including agonists, antagonists, and modulators.
A: Hypoxic conditions might affect the function and expression of the CHRM3 receptor, thereby impacting cell survival, proliferation, and migration. The specific effects need to be determined experimentally.
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