Cat.No. : CSC-RG0021 Host Cell: U2OS
| Cat. No. | CSC-RG0021 |
| Background | The endothelins and their receptors are referred as the endothelin (ET) axis and this axis has been implicated in diverse tumours. Endothelin B receptor (ETB) may oppose cancer advance by helping apoptosis and clearing ET-1; however, it has recently been involved in the progress of some tumour as melanomas or oligodendrogliomas. The multigenic disorder, Hirschsprung disease type 2, is due to mutation in endothelin receptor type B gene. |
| Gene | EDNRB |
| Gene Species | Homo sapiens (Human) |
| Alias | EDNRB, ABCDS, ET-B, ET-BR, ETB, ETBR, ETRB, HSCR, HSCR2, WS4A |
| Host Cell | U2OS |
| Host Cell Species | Homo sapiens (Human) |
| Morphology | Epithelial |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human EDNRB Stable Cell Line-U2OS can contribute to our understanding of the role of EDNRB in osteosarcoma progression by facilitating in-depth studies on the molecular mechanisms, signaling pathways, and downstream effects of EDNRB activation in these cancer cells. This knowledge can provide valuable insights into potential therapeutic strategies.
A: Yes, the Human EDNRB Stable Cell Line-U2OS can be employed to study the effects of EDNRB agonists and antagonists on cellular behavior. Researchers can utilize this cell line to investigate how various ligands or compounds interact with EDNRB and modulate cell signaling, proliferation, migration, or other functional responses.
A: As of my last training data update in January 2022, there were no specific studies or publications mentioned involving the Human EDNRB Stable Cell Line-U2OS. However, it is essential to consult the most recent scientific literature for any updates or relevant research.
A: The Human EDNRB Stable Cell Line-U2OS offers the advantage of stable and consistent expression of EDNRB, enabling researchers to obtain reliable and reproducible results in their experiments. It provides a relevant human osteosarcoma cellular context for studying EDNRB, which can be particularly valuable for cancer research and drug development.
A: The creation of the Human EDNRB Stable Cell Line-U2OS aims to establish a dependable cellular model for studying the Endothelin B receptor (EDNRB) in human osteosarcoma (U2OS) cells. This facilitates investigations into the functional roles and signaling pathways associated with EDNRB in this specific cellular context.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.
Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Copyright © Creative Biogene. All rights reserved.
