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Cat.No. : CSC-RG0020 Host Cell: U2OS
| Cat. No. | CSC-RG0020 |
| Background | The gastrin-releasing peptide receptor (GRPR), properly known as Bombesin Receptor 2 (BB2) is a G protein-coupled receptor whose endogenous ligand is gastrin releasing peptide. The bombesin receptors are a group of Gprotein coupled receptors : BB1 or Neuromedin B receptor (NMBR); BB2,or Gastrin-releasing peptide receptor (GRPR); BB3, or Bombesin-like receptor 3 (BRS3) |
| Gene | GRPR |
| Gene Species | Homo sapiens (Human) |
| Alias | GRPR, GRP-R |
| Host Cell | U2OS |
| Species | Homo sapiens (Human) |
| Morphology | Epithelial |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The creation of a Human GRPR Stable Cell Line-U2OS serves the purpose of establishing a reliable and consistent cellular model for studying the Gastrin-Releasing Peptide Receptor (GRPR) in human osteosarcoma (U2OS) cells. This allows researchers to investigate the role and function of GRPR in this specific cellular context.
A: The Human GRPR Stable Cell Line-U2OS is generated through a process where U2OS cells are transfected with a plasmid containing the GRPR gene, followed by selection and maintenance of stable clones using appropriate selection markers or antibiotics. These cells are then cultured and maintained in suitable growth media supplemented with the necessary selective agents.
A: The advantages of using the Human GRPR Stable Cell Line-U2OS include its stable expression of GRPR, which allows for consistent and reproducible results in experiments. It also provides a human osteosarcoma cellular context for studying GRPR, which can be relevant for cancer research and drug development.
A: The Human GRPR Stable Cell Line-U2OS can be used for various research applications, including investigating the signaling pathways, downstream effects, and functional roles of GRPR in osteosarcoma cells. It can also be employed in drug screening, receptor ligand binding studies, and exploring potential therapeutic targets.
A: The key molecular characteristics of the Human GRPR Stable Cell Line-U2OS include stable integration of the GRPR gene into the cellular genome, resulting in sustained expression of the GRPR receptor on the cell surface. These cells may also have specific markers or reporter genes for monitoring GRPR activity.
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