Human GRPR Stable Cell Line-U2OS

Name: Human GRPR Stable Cell Line-U2OS
SKU: CSC-RG0020
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0020

Host Cell : U2OS Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0020
Background	The gastrin-releasing peptide receptor (GRPR), properly known as Bombesin Receptor 2 (BB2) is a G protein-coupled receptor whose endogenous ligand is gastrin releasing peptide. The bombesin receptors are a group of Gprotein coupled receptors : BB1 or Neuromedin B receptor (NMBR); BB2,or Gastrin-releasing peptide receptor (GRPR); BB3, or Bombesin-like receptor 3 (BRS3)
Target Gene	GRPR
Gene Species	Homo sapiens (Human)
Abbr	U2OS-HuGRPR
Alias	GRPR, GRP-R
Host Cell	U2OS
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	GRPR gastrin-releasing peptide receptor [ Homo sapiens ]
Gene Symbol	GRPR
Gene Description	gastrin-releasing peptide receptor
GeneID	2925
Uni ProtID	P30550
mRNA Refseq	NM_005314.2
Protein Refseq	NP_005305.1
Chromosome Location	Xp22.2
Function	G-protein coupled peptide receptor activity; bombesin receptor activity; neuropeptide binding;
Pathway	Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; GPCRs, Class A Rhodopsin-like, organism-specific biosystem;
MIM	305670

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What is the purpose of creating a Human GRPR Stable Cell Line-U2OS?

A: The creation of a Human GRPR Stable Cell Line-U2OS serves the purpose of establishing a reliable and consistent cellular model for studying the Gastrin-Releasing Peptide Receptor (GRPR) in human osteosarcoma (U2OS) cells. This allows researchers to investigate the role and function of GRPR in this specific cellular context.

How is the Human GRPR Stable Cell Line-U2OS generated and maintained?

A: The Human GRPR Stable Cell Line-U2OS is generated through a process where U2OS cells are transfected with a plasmid containing the GRPR gene, followed by selection and maintenance of stable clones using appropriate selection markers or antibiotics. These cells are then cultured and maintained in suitable growth media supplemented with the necessary selective agents.

What are the advantages of using the Human GRPR Stable Cell Line-U2OS for GRPR-related research?

A: The advantages of using the Human GRPR Stable Cell Line-U2OS include its stable expression of GRPR, which allows for consistent and reproducible results in experiments. It also provides a human osteosarcoma cellular context for studying GRPR, which can be relevant for cancer research and drug development.

What specific research applications can the Human GRPR Stable Cell Line-U2OS be used for?

A: The Human GRPR Stable Cell Line-U2OS can be used for various research applications, including investigating the signaling pathways, downstream effects, and functional roles of GRPR in osteosarcoma cells. It can also be employed in drug screening, receptor ligand binding studies, and exploring potential therapeutic targets.

What are the key molecular characteristics of the Human GRPR Stable Cell Line-U2OS?

A: The key molecular characteristics of the Human GRPR Stable Cell Line-U2OS include stable integration of the GRPR gene into the cellular genome, resulting in sustained expression of the GRPR receptor on the cell surface. These cells may also have specific markers or reporter genes for monitoring GRPR activity.

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