Human ADCYAP1R1 Stable Cell Line-HEK293

Name: Human ADCYAP1R1 Stable Cell Line-HEK293
SKU: CSC-RG0011
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0011

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

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Gene Information

Cat. No.	CSC-RG0011
Background	Pituitary adenylate cyclase-activating polypeptide type I receptor, also known as PAC1 is a protein that in humans is encoded by the ADCYAP1R1 gene. ADCYAP1R1 is a membrane-associated protein and shares significant homology with members of the glucagon/secretin receptor family. This receptor binds pituitary adenylate cyclase activating peptide (PACAP) mediating several biological activities and it is positively coupled to adenylate cyclase. ADCYAP1R1 is expressed in the adrenal medulla, pancreatic acini, uterus, myenteric plexus and brain.
Target Gene	ADCYAP1R1
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuADCYAP1R1
Alias	ADCYAP1R1, PAC1, PAC1R, PACAPR, PACAPRI
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	Homo sapiens adenylate cyclase activating polypeptide 1 (pituitary) receptor type I DNA.
Gene Symbol	ADCYAP1R1
Synonyms	PAC1, PAC1R, PACAPR, PACAPRI
m RNA Refseq	BC117116

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Customer Q&As

What is the gene editing efficiency of the Human ADCYAP1R1 Stable Cell Line-HEK293?

A: The gene editing efficiency in the Human ADCYAP1R1 Stable Cell Line-HEK293 depends on the editing tool used (like CRISPR/Cas9) and the specific target sequence. The efficiency can vary from moderate to very high but needs to be determined experimentally.

How can the expression level of ADCYAP1R1 protein be quantified in the Human ADCYAP1R1 Stable Cell Line-HEK293?

A: The expression level of ADCYAP1R1 protein can be quantified through Western Blot or Enzyme-Linked Immunosorbent Assay (ELISA). These methods provide quantitative expression level information.

What drugs or compounds is the Human ADCYAP1R1 Stable Cell Line-HEK293 particularly sensitive to?

A: This needs to be determined through drug screening experiments. ADCYAP1R1 is related to multiple signaling pathways, so it might be sensitive to drugs affecting these pathways, such as agonists or antagonists of the G-protein-coupled receptor (GPCR) pathway.

What is the most effective way to induce gene silencing in the Human ADCYAP1R1 Stable Cell Line-HEK293?

A: Using small interfering RNA (siRNA) or short hairpin RNA (shRNA) are common methods to induce gene silencing. Selecting sequences specific to the ADCYAP1R1 gene can improve silencing efficiency.

What impact do mutations in ADCYAP1R1 have on cell function in the Human ADCYAP1R1 Stable Cell Line-HEK293?

A: Mutations in ADCYAP1R1 could affect cell signaling, proliferation, and survival. The specific impacts need to be determined through functional assays like cell proliferation tests, apoptosis analysis, etc.

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