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Cat.No. : CSC-RI0047 Host Cell: HEK293
| Cat. No. | CSC-RI0047 |
| Description | This cell line is engineered to overexpress human SCN1A. |
| Background | The voltage gated sodium channel isoform Nav1.1 is involved with axon potential initiation and repetitive neuronal firing as well as the contraction-excitation coupling of cardiac myocytes. Nav1.1 is the target of antiepileptic drugs and the probable target causing side effects of centrally active local anaesthetics. It is detected cardiac myocytes as well as in the cell bodies of central neurons. |
| Gene | SCN1A |
| Gene Species | Homo sapiens (Human) |
| Alias | SCN1A, EIEE6, FEB3, FEB3A, FHM3, GEFSP2, HBSCI, NAC1, Nav1.1, SCN1, SMEI |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Morphology | Epithelial |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The cell line was established by stably transfecting the human SCN1A gene (sodium channel subunit alpha 1A) into HEK293 cells.
A: As SCN1A gene mutations are closely related to certain types of epilepsy, using this cell line can help researchers understand the molecular mechanisms of epilepsy more deeply.
A: Special attention should be paid to the impact of drugs on SCN1A channel activity and the resulting changes in the cell's electrophysiological properties.
A: Although SCN1A is mainly associated with neurological diseases, it can also be used to study the function of sodium channels in cardiac pathologies.
A: It can be used to assess the potential impact of drugs on SCN1A channels in the nervous system, especially in the treatment of epilepsy and other neurological disorders.
A: Consideration should be given to how to simulate the in vivo environment, such as using appropriate ion concentrations and electrophysiological techniques, to accurately assess the function of the SCN1A channel.
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