Human SCN1A Stable Cell Line-HEK293

Name: Human SCN1A Stable Cell Line-HEK293
SKU: CSC-RI0047
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0047

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0047
Description	This cell line is engineered to overexpress human SCN1A.
Background	The voltage gated sodium channel isoform Nav1.1 is involved with axon potential initiation and repetitive neuronal firing as well as the contraction-excitation coupling of cardiac myocytes. Nav1.1 is the target of antiepileptic drugs and the probable target causing side effects of centrally active local anaesthetics. It is detected cardiac myocytes as well as in the cell bodies of central neurons.
Target Gene	SCN1A
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuSCN1A
Alias	SCN1A, EIEE6, FEB3, FEB3A, FHM3, GEFSP2, HBSCI, NAC1, Nav1.1, SCN1, SMEI
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	SCN1A sodium channel, voltage-gated, type I, alpha subunit [ Homo sapiens ]
Gene Symbol	SCN1A
Synonyms	FEB3; FHM3; NAC1; SCN1; SMEI; EIEE6; FEB3A; HBSCI; GEFSP2; Nav1.1
Gene Description	sodium channel, voltage-gated, type I, alpha subunit
GeneID	6323
Uni ProtID	P35498
mRNA Refseq	NM_006920.4
Protein Refseq	NP_008851.3
Chromosome Location	2q24.3
Function	voltage-gated sodium channel activity;
Pathway	Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Dopaminergic synapse, organism-specific biosystem; Dopaminergic synapse, conserved biosystem; Interaction between L1 and Ankyrins, organism-specific biosystem; L1CAM interactions, organism-specific biosystem;
MIM	182389

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Q & A

Customer Reviews

Customer Q&As

How was the Human SCN1A Stable Cell Line-HEK293 established?

A: The cell line was established by stably transfecting the human SCN1A gene (sodium channel subunit alpha 1A) into HEK293 cells.

What are the specific advantages of using the Human SCN1A Stable Cell Line-HEK293 in epilepsy research?

A: As SCN1A gene mutations are closely related to certain types of epilepsy, using this cell line can help researchers understand the molecular mechanisms of epilepsy more deeply.

What factors should be considered when conducting drug testing with the Human SCN1A Stable Cell Line-HEK293?

A: Special attention should be paid to the impact of drugs on SCN1A channel activity and the resulting changes in the cell's electrophysiological properties.

What are the applications of the Human SCN1A Stable Cell Line-HEK293 in cardiology research?

A: Although SCN1A is mainly associated with neurological diseases, it can also be used to study the function of sodium channels in cardiac pathologies.

What are the potential uses of the Human SCN1A Stable Cell Line-HEK293 in neuropharmacological research?

A: It can be used to assess the potential impact of drugs on SCN1A channels in the nervous system, especially in the treatment of epilepsy and other neurological disorders.

What special experimental designs should be considered when using the Human SCN1A Stable Cell Line-HEK293 in neuroscience experiments?

A: Consideration should be given to how to simulate the in vivo environment, such as using appropriate ion concentrations and electrophysiological techniques, to accurately assess the function of the SCN1A channel.

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