Human EDNRB-SNAP Stable Cell Line-HEK293

Name: Human EDNRB-SNAP Stable Cell Line-HEK293
SKU: CSC-RG0048
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0048

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

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Gene Information

Cat. No.	CSC-RG0048
Background	The protein encoded by this gene is a G protein-coupled receptor which activates a phosphatidylinositol-calcium second messenger system. Its ligand, endothelin, consists of a family of three potent vasoactive peptides: ET1, ET2, and ET3. Studies suggest that the multigenic disorder, Hirschsprung disease type 2, is due to mutations in the endothelin receptor type B gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Target Gene	EDNRB
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuEDNRB-SNAP
Alias	EDNRB, ET-B, ETBR, ETRB, HSCR, WS4A, BCDS, ET-BR, HSCR2
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	Homo sapiens endothelin receptor type B DNA.
Gene Symbol	EDNRB
Synonyms	ETB, ET-B, ETBR, ETRB, HSCR, WS4A, ABCDS, ET-BR, HSCR2
m RNA Refseq	BC014472

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How is the Human EDNRB-SNAP Stable Cell Line-HEK293 constructed through genetic engineering?

A: The Human EDNRB-SNAP Stable Cell Line-HEK293 is constructed by fusing the gene encoding EDNRB (endothelin receptor B) with a SNAP tag (a tag used for protein labeling) and then introducing this fusion gene into HEK293 cells via transfection. Through selection and cloning, a cell line stably expressing the EDNRB-SNAP fusion protein is ultimately obtained.

What is the role of the Human EDNRB-SNAP Stable Cell Line-HEK293 in studying endothelin signaling?

A: This cell line serves as a model system to study the function of EDNRB in endothelin signaling. By observing the localization and dynamics of SNAP-tagged EDNRB within the cells, researchers can better understand how endothelin affects cellular behaviors such as vasoconstriction and angiogenesis.

What is the application of the Human EDNRB-SNAP Stable Cell Line-HEK293 in drug screening?

A: This cell line can be used for high-throughput screening (HTS) and drug discovery, particularly for potential drugs targeting the endothelin receptor B. By monitoring changes in the activity of SNAP-tagged EDNRB, compounds can be assessed for their effects on EDNRB function, facilitating the screening of potential therapeutic candidates.

What is the value of the Human EDNRB-SNAP Stable Cell Line-HEK293 in studying the regulatory mechanisms of endothelin receptor B?

A: This cell line provides a platform for studying the regulation of endothelin receptor B, allowing scientists to investigate the expression, modification, and interactions of EDNRB with other signaling molecules. This is crucial for understanding endothelin signaling in cardiovascular and neurological diseases.

What role does the Human EDNRB-SNAP Stable Cell Line-HEK293 play in studying the disease relevance of endothelin receptor B?

A: This cell line aids in investigating the role of endothelin receptor B in various diseases, including hypertension, heart disease, and neurodegenerative disorders. By simulating disease states at the cellular level, it enables the exploration of EDNRB's function in disease progression and the development of targeted therapeutic strategies.

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