Cat.No. : CSC-RG0048 Host Cell: HEK293
| Cat. No. | CSC-RG0048 |
| Background | The protein encoded by this gene is a G protein-coupled receptor which activates a phosphatidylinositol-calcium second messenger system. Its ligand, endothelin, consists of a family of three potent vasoactive peptides: ET1, ET2, and ET3. Studies suggest that the multigenic disorder, Hirschsprung disease type 2, is due to mutations in the endothelin receptor type B gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Gene | EDNRB |
| Gene Species | Homo sapiens (Human) |
| Alias | EDNRB, ET-B, ETBR, ETRB, HSCR, WS4A, BCDS, ET-BR, HSCR2 |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Morphology | Epithelial |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human EDNRB-SNAP Stable Cell Line-HEK293 is constructed by fusing the gene encoding EDNRB (endothelin receptor B) with a SNAP tag (a tag used for protein labeling) and then introducing this fusion gene into HEK293 cells via transfection. Through selection and cloning, a cell line stably expressing the EDNRB-SNAP fusion protein is ultimately obtained.
A: This cell line serves as a model system to study the function of EDNRB in endothelin signaling. By observing the localization and dynamics of SNAP-tagged EDNRB within the cells, researchers can better understand how endothelin affects cellular behaviors such as vasoconstriction and angiogenesis.
A: This cell line can be used for high-throughput screening (HTS) and drug discovery, particularly for potential drugs targeting the endothelin receptor B. By monitoring changes in the activity of SNAP-tagged EDNRB, compounds can be assessed for their effects on EDNRB function, facilitating the screening of potential therapeutic candidates.
A: This cell line provides a platform for studying the regulation of endothelin receptor B, allowing scientists to investigate the expression, modification, and interactions of EDNRB with other signaling molecules. This is crucial for understanding endothelin signaling in cardiovascular and neurological diseases.
A: This cell line aids in investigating the role of endothelin receptor B in various diseases, including hypertension, heart disease, and neurodegenerative disorders. By simulating disease states at the cellular level, it enables the exploration of EDNRB's function in disease progression and the development of targeted therapeutic strategies.
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