Mouse Npsr1 Stable Cell Line-HEK293T

Name: Mouse Npsr1 Stable Cell Line-HEK293T
SKU: CSC-RG0324
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0324

Host Cell : HEK293T Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0324
Background	Neuropeptide S (NPS) receptor 1 is also known as GPR154 or G proteincoupled receptor for asthma susceptibility (GPRA). NPS receptor is widely expressed in the brain, with highest levels found in hypothalamus, amygdala, endopiriform nucleus, cortex, subiculum and nuclei of the thalamic midline. Central administration of NPS promotes behavioral arousal and suppresses all stages of sleep in rodents. Furthermore, NPS was found to produce anxiolytic-like effects in a battery of four different tests that measure behavioral responses of rodents to novelty or stress.
Target Gene	Npsr1
Gene Species	Mus musculus (Mouse)
Abbr	HEK293T-MouseNpsr1
Alias	NPSR1, GPRA, MVTR, VRR1, PGR14, Gpr154
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	Npsr1 neuropeptide S receptor 1 [ Mus musculus ]
Gene Symbol	Npsr1
Synonyms	GPRA; MVTR; VRR1; PGR14; Gpr154; 9330128H10Rik
Gene Description	neuropeptide S receptor 1
Gene ID	319239
Uni Prot ID	A1KXK3
m RNA Refseq	NM_175678.3
Protein Refseq	NP_783609.1
Chromosome Location	9 A3-A4; 9
Function	G-protein coupled receptor activity; signal transducer activity; vasopressin receptor activity;
Pathway	Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem; G alpha (s) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; Gastrin-CREB signalling pathway via PKC and MAPK, organism-specific biosystem; Non-odorant GPCRs, organism-specific biosystem;

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