Human NPSR1 variant 2 Stable Cell Line-HEK293T

Name: Human NPSR1 variant 2 Stable Cell Line-HEK293T
SKU: CSC-RG0326

Cat.No. : CSC-RG0326 Host Cell: HEK293T

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Cat. No.	CSC-RG0326
Background	Neuropeptide S (NPS) receptor 1 is also known as GPR154 or G protein-coupled receptor for asthma susceptibility (GPRA). NPS receptor is widely expressed in the brain, with highest levels found in hypothalamus, amygdala, endopiriform nucleus, cortex, subiculum and nuclei of the thalamic midline. Central administration of NPS promotes behavioral arousal and suppresses all stages of sleep in rodents. Furthermore, NPS was found to produce anxiolytic-like effects in a battery of four different tests that measure behavioral responses of rodents to novelty or stress.
Gene	NPSR1
Gene Species	Homo sapiens (Human)
Alias	NPSR1, GPR154, GPRA, PGR14, NPSR, VRR1, ASRT2
Host Cell	HEK293T
Species	Homo sapiens (Human)
Morphology	Epithelial
Stability	Validated for at least 10 passages
Application	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Shipping	Dry ice
Storage	Liquid nitrogen

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm² flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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