Human NPSR1 variant Asn107Ile cDNA (SNP591694 A>T; refSNP ID rs324981) Stable Cell Line-HEK293T

Name: Human NPSR1 variant Asn107Ile cDNA (SNP591694 A>T; refSNP ID rs324981) Stable Cell Line-HEK293T
SKU: CSC-RG0327
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0327

Host Cell : HEK293T Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0327
Target Gene	NPSR1
Gene Species	Homo sapiens (Human)
Abbr	HEK293T-HuNPSR1 variant Asn107Ile cDNA (SNP591694 A>T; refSNP ID rs324981)
Alias	NPSR1, GPR154, GPRA, PGR14, NPSR, VRR1, ASRT2
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	NPSR1 neuropeptide S receptor 1 [ Homo sapiens ]
Gene Symbol	NPSR1
Synonyms	GPRA; NPSR; VRR1; ASRT2; PGR14; GPR154
Gene Description	neuropeptide S receptor 1
Gene ID	387129
Uni Prot ID	Q6W5P4
m RNA Refseq	NM_207172.1
Protein Refseq	NP_997055.1
Chromosome Location	7p14.3
Function	vasopressin receptor activity;
Pathway	Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem; G alpha (s) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; Gastrin-CREB signalling pathway via PKC and MAPK, organism-specific biosystem; Peptide ligand-binding receptors, organism-specific biosystem;
MIM	608595

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Neuropeptide S (NPS) receptor 1 is also known as GPR154 or G proteincoupled receptor for asthma susceptibility (GPRA). NPS receptor is widely expressed in the brain, with highest levels found in hypothalamus, amygdala, endopiriform nucleus, cortex, subiculum and nuclei of the thalamic midline. Central administration of NPS promotes behavioral arousal and suppresses all stages of sleep in rodents. Furthermore, NPS was found to produce anxiolytic-like effects in a battery of four different tests that measure behavioral responses of rodents to novelty or stress. A number of polymorphisms in the human NPS receptor gene have been identified and a specific set of these polymorphisms was linked to an increased susceptibility for asthma and potentially other forms of allergy that are characterized by high IgE serum levels in Finnish and Canadian asthma patients. One of the single nucleotide polymorphisms Asn107Ile in the human receptor results in a gain-of-function characterized by an increase in agonist potency without changing binding affinity.

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