Human BDKRB2 Stable Cell Line-U2OS

Name: Human BDKRB2 Stable Cell Line-U2OS
SKU: CSC-RG0006
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0006

Host Cell : U2OS Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0006
Background	Bradykinin receptor B2 is a G-protein coupled receptor for Bradykinin, encoded by the BDKRB2 gene in humans. The B2 receptor is a G protein-coupled receptor that stimulates phospholipase C to increase intracellular free calcium. The 9 amino acid Bradykinin peptide elicits many responses including vasodilation, edema, smooth muscle spasm and pain fiber stimulation.
Target Gene	BDKRB2
Gene Species	Homo sapiens (Human)
Abbr	U2OS-HuBDKRB2
Alias	BDKRB2, B2R, BK-2, BK2, BKR2, BRB2
Host Cell	U2OS
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	BDKRB2 bradykinin receptor B2 [ Homo sapiens ]
Gene Symbol	BDKRB2
Synonyms	B2R; BK2; BK-2; BKR2; BRB2
Gene Description	bradykinin receptor B2
GeneID	624
Uni ProtID	P30411
mRNA Refseq	NM_000623.3
Protein Refseq	NP_000614.1
Chromosome Location	14q32.1-q32.2
Function	bradykinin receptor activity; phosphatidylinositol phospholipase C activity; protease binding; protein binding; protein heterodimerization activity; type 1 angiotensin receptor binding;
Pathway	ACE Inhibitor Pathway, organism-specific biosystem; Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Chagas disease (American trypanosomiasis), organism-specific biosystem; Chagas disease (American trypanosomiasis), conserved biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem;
MIM	113503

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Customer Q&As

What is the potential use of the Human BDKRB2 Stable Cell Line-U2OS in cardiovascular disease research?

A: This cell line can be used to study the role of BDKRB2 in regulating vascular tone and blood pressure, particularly in researching cardiovascular diseases associated with it, such as hypertension and heart failure. By performing drug screening and functional analysis on these cells, scientists can better understand the mechanisms by which BDKRB2 contributes to the development of cardiovascular diseases.

How can the Human BDKRB2 Stable Cell Line-U2OS be utilized for drug screening to discover new vasodilators?

A: By applying potential vasodilators to these cells, researchers can monitor the effects of drugs on the signaling pathways mediated by BDKRB2, such as endothelium-dependent vasodilation. This screening can help identify new drugs for treating hypertension and other vascular-related diseases.

How can the role of BDKRB2 in cell proliferation be studied using the Human BDKRB2 Stable Cell Line-U2OS?

A: By treating these cells with drugs or performing gene knockout experiments, the impact of BDKRB2 on cell cycle arrest can be observed. This contributes to understanding the role of BDKRB2 in cell proliferation and tumorigenesis.

What are the applications of the Human BDKRB2 Stable Cell Line-U2OS in drug metabolism studies?

A: The cell line can be used to study the role of BDKRB2 in drug metabolism, such as analyzing the metabolic products and pathways of drugs in these cells, as well as the impact of BDKRB2 on the activity of drug-metabolizing enzymes.

How is the function of the BDKRB2 receptor verified in the Human BDKRB2 Stable Cell Line-U2OS?

A: Functional verification typically involves activating the BDKRB2 receptor with drugs or agonists and monitoring downstream effects, such as vasodilation or changes in endothelial cell function. Additionally, gene knockout or RNA interference techniques can be used to suppress the expression of BDKRB2, observing the impact of these manipulations on cell function.

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