Cat.No. : CSC-RG0006 Host Cell: U2OS
| Cat. No. | CSC-RG0006 |
| Background | Bradykinin receptor B2 is a G-protein coupled receptor for Bradykinin, encoded by the BDKRB2 gene in humans. The B2 receptor is a G protein-coupled receptor that stimulates phospholipase C to increase intracellular free calcium. The 9 amino acid Bradykinin peptide elicits many responses including vasodilation, edema, smooth muscle spasm and pain fiber stimulation. |
| Gene | BDKRB2 |
| Gene Species | Homo sapiens (Human) |
| Alias | BDKRB2, B2R, BK-2, BK2, BKR2, BRB2 |
| Host Cell | U2OS |
| Host Cell Species | Homo sapiens (Human) |
| Morphology | Epithelial |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: This cell line can be used to study the role of BDKRB2 in regulating vascular tone and blood pressure, particularly in researching cardiovascular diseases associated with it, such as hypertension and heart failure. By performing drug screening and functional analysis on these cells, scientists can better understand the mechanisms by which BDKRB2 contributes to the development of cardiovascular diseases.
A: By applying potential vasodilators to these cells, researchers can monitor the effects of drugs on the signaling pathways mediated by BDKRB2, such as endothelium-dependent vasodilation. This screening can help identify new drugs for treating hypertension and other vascular-related diseases.
A: By treating these cells with drugs or performing gene knockout experiments, the impact of BDKRB2 on cell cycle arrest can be observed. This contributes to understanding the role of BDKRB2 in cell proliferation and tumorigenesis.
A: The cell line can be used to study the role of BDKRB2 in drug metabolism, such as analyzing the metabolic products and pathways of drugs in these cells, as well as the impact of BDKRB2 on the activity of drug-metabolizing enzymes.
A: Functional verification typically involves activating the BDKRB2 receptor with drugs or agonists and monitoring downstream effects, such as vasodilation or changes in endothelial cell function. Additionally, gene knockout or RNA interference techniques can be used to suppress the expression of BDKRB2, observing the impact of these manipulations on cell function.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.
Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Copyright © Creative Biogene. All rights reserved.
